Danh Sách 69 Chỉ Thị Ssr Sử Dụng Để Phát Triển Gen Bố Mẹ Và Quần Thể F2 Trong Nghiên Cứu



TT


Tên chỉ thị

Trình tự mồi

Nhiệt độ gắn

mồi

Kích thước sản phẩm

PCR

Mồi xuôi

Mồi ngược

179

CMTm230

GGATGCCATATATCTGAATCCTG

CGAAATACCGCTGCAAAAG

55

119-130

180

CMTp97

CCACACACCAATCGTTGAAG

CGCAGAATCTCGAAACACAA

54

163-169

181

CMTp130

GCCCATTTCTGGAGAGATAGTA

GAGGAGAGATGCAGAGCAAC

55

164-172

182

CMTp169

TGCAGTGAATGGAAACCTGT

TCCGGCATTATAATCAGAACC

59

153-161

183

CMTp225

CCATCTTTCCACCCTCAATG

AGCTGAAGTGCCTTCTTGGA

50

146-156

184

CMTp38

AGGATTTCAGGTATGGTCGTG

AGACGTTCCCTCCCCTCT

56

160

185

CMTp61

AAGGGTTCGTCGGAGAAACT

CAACGACTCCCAGCACCTAT

56

118

186

CMTp74

ACCCCTCTTGTTCTTCAAGG

TTACTCCCATTTTCCCCATT

58

184

187

CMTp75

TGGGGAAAATGGGAGTAAGAG

TGCTTGCGTTGGCTTACATA

56

118

188

CMTp76

TGAAACAGTCATCTCTTTTGTTGA

TCTCTGGGTGTGTTTCTTGC

46

175

189

CMTmC73

TTTTCACACCGAATGAGCTTG

GCCCACTAATTCTAGGGTTTGC

45

215

190

CMTmC66

TCAACCCAACATTTCTCTCC

AGAAGAGGCTGAGGAGTCG

59

175

191

CMTmC65

ACAGAGCATGATCCAACCTG

CCATTGGGCAGATCCTTC

55

171

192

CMTmC58

TGAATTCTCCTTCCCCTCGT

CCCTCCGAAGAAAGAGAAGC

54

100

193

CMTmC57

AACTAGGGTTGTGTCTTAGAAAAATCA

TGATGATGCTAATGATGTTAATGATG

55

185

194

CMTp5

GGGTCTATCTCTGTGTTGACTGG

AGGACGCATGGAGTAGCAAT

55

178

195

CMTmC50

CAGGGGCTCTGATCTGAAAG

TTTGATGCATCTCTTGGAAGG

60

132

196

CMTmC47

GAGATTCGGTTCAGGCAATG

CTGGCTGGCTTCGCTACTT

57

122

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Nghiên cứu ứng dụng chỉ thị phân tử phục vụ chọn tạo giống bí đỏ Cucurbita spp. có hàm lượng chất khô cao - 20



TT


Tên chỉ thị

Trình tự mồi

Nhiệt độ gắn

mồi

Kích thước sản phẩm

PCR

Mồi xuôi

Mồi ngược

197

CMTp221

CAAACTCTTTCTTCATGGAACG

TGAAACTCAGCGAAAACTCG

56

111

198

CMTp132

CCATTTCCATTTCCATTTCA

AGGTTAGAAACAGGGGGAATC

46

151

199

CMTm233

CCCTTCTCGCTGATTTCAAG

CAGCGCAACAGCATTACATC

59

136

200

CMTmC15

TATGTAGCGCTCTGCACAAT

CCTCAATGTAATGTTTTGAATCC

55

113

201

CMTp188

CCGAAGGTTAGCAGACCCTTA

AAGGACCTCATGCATTTTGC

47

147

202

CMTmC21

GTTTTCCCGAGGAAGAGGAG

ACCTTATATTCTGGTCTGCCACA

55

112

203

CMTp18

TGACATCACTCCGGCAACTC

ACACCTTCGCTTCCGACATC

51

158

204

CMTm187

ATCGGTGAGTCCCAAAAATG

ATCACAAAGCGGGAAAACAC

52

128

205

CMTm49

TCCCCATTCATTCATTCATC

GGAATCTGAGGCTTTTGAGG

50

100

206

CMTp133

TGCTCTCTTTGAATTCAGCAT

AGGGATCTTCCATTTCCAAT

56

113

207

CMTm158

CCCAGCTATGAAGGTCTCTG

AAAAGCACAAAAGAAACACCA

55

80

208

CMTp125

CTTGTTCCGCAGCATCAG

AGTGAGAGGGAGACGCAAAG

55

115

209

CMTp127

TTCCTCTGTTTCCGTCAATG

CACAAAAGGGGTTTGAGGTT

55

112

210

CMTp107

CGATGATGAACAGGAAGACG

TCACATCCATTCCCCTCTCT

59

109

211

CMTp158

CCGTAGAGATGTCAGAGACAAGG

AGGGATGCTCATCACACCTC

45

134

212

CMTm259

ATGGAGACGCGCAAGTAGAT

ACCTCGAGGAAGCAAAAATG

56

169

213

CMTp176

CCTGGACTTCCACATCAGTT

ACTACGTGTCTCTGCAGGAAG

58

111

214

CMTp33

AATGCGTTTGAACAAAGCTG

GGCTGTTAAAATTTCCTCGATT

46

171



TT


Tên chỉ thị

Trình tự mồi

Nhiệt độ gắn

mồi

Kích thước sản phẩm

PCR

Mồi xuôi

Mồi ngược

215

CMTm65

GAACTTCGTGTGTGCGTGTC

TTGCTGGAACTTCCTCTCGT

57

97

216

CMTp69

ATACTTGCTCCCCAAGTTTA

AAATAAAAAGACAACGTAATGGT

60

70

217

CMTp68

CACACCCATTTCATTTTGACC

ATTGATTGGGACGTGAGGAA

47

189

218

CMTp36

ATTGATTGGGACGTGAGGAA

TTCAGCTTCCCGGATTATGG

59

151

219

CMTm111

CTCCATTCCCATGGCTTC

CCATGAGCTTGAGAGAGGTG

46

110

220

CMTp145

ACAGCCCCATTTCTTACCG

TCAAGAACTGAGGGGATTGG

51

100

221

CMTp138

AAAGGTTTCCACATCCTTG

GAAAAGGAAAAAGTGTTCAAAG

59

103

222

CMTm221

CAATAAGATAGCTCTCACGTTGC

TGCCTAGTTATCGCGACTTC

46

108

223

CMTm183

CTTTGCATTGCCACTCTTCT

TGAGGGAAATTGAATGTGGT

60

115

224

CMTmC14

AAGCCGTGATGGACAACATT

CCACCACCCCTGATTTTAACT

59

129

225

CMTm66

ACGCGTTGTTGTTAGTGTGG

GGGGATTTATGAACCCAACAT

45

126

226

CMTm9

GCCCAGAAGACAAAAGTTCG

TTTTTGTGTGCGTGTGTGG

58

158

227

CMTp248

GATCGAATTGGGGCTTCTT

CCTCCGTACATCCCCAGA

53

154

228

CMTm170

TCAGACCCACTCCCATGAAC

AGCGAACACGTGAAAACGTC

58

113

229

CMTm130

CCCTCCACCACCTCCTTAG

CAGGGGGATCATAAAAGTGG

55

110

230

CMTm172

CATTGGAGTGCGTGAAATTG

GTGAGTGGTCAAAGGGCATT

59

115

231

CMTp63

CTTCGTCGACACCAATTCC

GAAGACGAAGATGACGTGGA

56

152

232

CMTm107

ACAAATGCCATGAAACACAACA

TGGTTGCTAGAGACCGTTGG

55

127



TT


Tên chỉ thị

Trình tự mồi

Nhiệt độ gắn

mồi

Kích thước sản phẩm

PCR

Mồi xuôi

Mồi ngược

233

CMTp201

AGGAGTGGTGGGCTAATACG

TGAAATTGAGGGAGGGAGAG

48

110

234

CMTm30

AGGTCCATATTTGACG

CAAACCATAACTTCCAG

55

161

235

CMTm11

TGGAAGGATTCTCCCACAGT

TACAATTTGACGTCCGCAAG

52

158

236

CMTmC67

ACATGTGGGTGCAATGAGG

CACCATTGTGGAAAATGAAACA

47

114

237

CMTm120

GCCAAAGGTTCCAAATGACA

TGATTTGCGCACAaACAAAC

56

121

238

CMTm126

TTTCATGTTATGATATTGTCAG

TCCAAAGCTTACGTTTACT

57

159

239

CMTmC34

CGACGAATCTTTTGCTCCTC

CTCAAATCCTCCCTCACTCG

50

107

240

CMTm131

TAGAGTTCGATCAATGCAA

GTTGATACTTCAACCACGTT

60

111

241

CMTm144

ACATGGGCATACCTCGAATC

CACCTGGCTGTTTTGTCTGA

59

150

242

CMTm206

AGGAACGGAGGGATAGAAAAGA

CCTTGTCCCCAAGTTGCATA

58

171

243

CMTm207

GGAAGAGAGAGATTGTAAGCACA

CCCTACACAACAGTGGCTCT

52

108

244

CMTm209

ATTAGAATCCCTTGGAGAACG

GCAATCGTTTTTCTGTTGGT

57

152

245

CMTm219

TTCAGAGGACATCTGCCATT

ATAGTTCTCGCTGGGCTTG

58

112

246

CMTm224

GTGGGTCGCTCCTTAAA

ATAGCGGATAAAGTGGACTG

52

120

247

CMTm252

CCCATTCCCCATTTCTTTCT

CAAATTCGATCGGCTTTACAC

59

101

248

CMTm261

GGTGGCCTCTGAACAATTTC

ACCTAACCAATGGGCATGAG

55

228

249

CMTm84

TATCACCAAACGTGCAT

AGGTTTTGAAGAACAAATTC

57

122

250

CMTm68

CGCCATCGTTGTTCTTCAC

AGATCCACAGCGATTTCGTC

47

109



TT


Tên chỉ thị

Trình tự mồi

Nhiệt độ gắn

mồi

Kích thước sản phẩm

PCR

Mồi xuôi

Mồi ngược

251

CMTm83

GGCATTTCTGAGAACAGCTT

ACGTTAGTTATGCTATTTTGTAGGC

55

111

252

CMTp182

CACGAAGATTTGATGGCCTTA

GGATTGGGATGGTGAAGATG

50

138

253

CMTp174

GCCGGAACCAGACTTCTC

CCCTCCCTTCCCATTAAAC

45

176

254

CMTp210

GTGGAAGTTACTGCGATTGG

GCAAAGAATGTCCTCAGCAG

50

117

255

CMTp247

GGGTGTGTTGAGGATTGGTT

ATCACATTTTCTCCCCACCA

56

123

256

CMTp46

TTCCCTTCTGCAGAGATGC T

CCATGCGCATAATTGTATCG

59

142

257

CMTp233

TTTCAAACGAGCACAAGCAC

GTTGCTCCAACTCGATCTTCA

60

104

258

CMTp236

CACATGCAATGAAATGATGG

AATTACCCGTTGAGACAGCA

55

82

259

CMTp241

ATCCACAAACAAGGCACCTC

GTAGTGGAGGCTCGGGTGTA

48

110

260

CMTp243

ATTCCGACTTGGGGATACCT

TGTCCTATCGCTTCGGAGAT

45

158

261

CMTp253

AGGTTGTGGAGGGTTTACTGG

ACAACAGGCCCAGAAACAAG

55

139

262

CMTp258

GGGCTACTTCCCCTAAAGATG

ACGATGCGTATTCACACTCAA

45

231

263

CMTp261

AAATGCCAGCCCAAATCAT

GATGGCTGCCACTTCCTCTA

46

187

264

CMTm17

CCTCTTTCAGGATTTCATTCAT

ACAAGCGATTATCTTACCGAGT

55

113

265

CMTm20

GTGGGCCATATCGATTCACT

CGAAAGTCGCAGAGAACACA

57

126

266

CMTm23

TGTATGGGGGAGTATTTTGG

TCAAGTCAACGAGTATCGTCAT

55

103

267

CMTm77

AACACTCGGCCACAACATC

CTCCTTGTAAAACGGGTTGC

59

189

268

CMTp48

TTTGCAGTACGCTGCAGAAC

GGATCCTTCTCGTGGTGGTA

55

228



TT


Tên chỉ thị

Trình tự mồi

Nhiệt độ gắn

mồi

Kích thước sản phẩm

PCR

Mồi xuôi

Mồi ngược

269

comp11018

GGGAGGCTACGAAGGAAAAG

CAATGGGGAAGTGGTAGGTG

55

nd

270

comp13350

GGACGAGGAGGTTTCAACAA

AAGGAAGCAAGAACACTCGG

56

nd

271

comp27618

TGAGCAGACCCTTGTTTTCC

GGTTGATGGGTGGAACTTTG

55

nd

272

comp28857

GACCAACCTCTTTCCCATCA

AAGGCGTAATAGCAGCTCCA

47

nd

273

comp40082

GGTGGTCCGGCTATCAACTA

TGACAGGGGATGAAAGGAAC

56

nd

274

comp41315

TTTGAAGTTGTTGTGCGGTG

ACTGTTGGCCGGTAGATTTG

57

nd

275

comp52235

GCCGATTGTTGAATATTTCG

TCATTGAGAGGGAGTCCCATA

50

nd

276

comp52664

CACTGTTTGGCAACGGTTTA

GATCCATGTGACAACCATGC

45

nd

277

comp64835

GTGGAGATCGTTGAAGGGAA

GAGCTTTCCACTCAGGCAAC

58

nd

278

comp66738

CGCCTTCATTGCAACATAAA

AGTGAGGGAAAGCAAAGCAA

56

nd

279

comp71199

CTGGGCACACTAGGGTCAAT

TTTATGCCGAGCAAACCAAT

57

nd

280

comp71344

GCTCTGGAGGATGAAACTCG

GGCTAACCCAGAAGGAAAGG

60

nd

281

comp71363

TGATGAGATTGAGAGCGGTG

AAGTGAATCCTTTCCGCCTT

48

nd

282

comp71542

AAATGCTCCTACCGAAGGGT

CAGCTTGAACATGATGCCAC

56

nd

283

comp72289

TGGTTGCTCATTGTCTTGCT

ATGCGCTATTTGCTTTCTCC

46

nd

284

comp72586

ATCACCACCGCAGGAACTAC

GGGCATCCCAACCTTTTATT

58

nd

285

CMTm89

ATAGGAATGTGCAGAGCTGAG

CAATATAGATACCGTTTTTCGAATC

56

nd

286

CMTmC1

AACGTCCTTACTGGCACACC

TTCCACAAGTTGTTTTGGTCAC

59

nd



TT


Tên chỉ thị

Trình tự mồi

Nhiệt độ gắn

mồi

Kích thước sản phẩm

PCR

Mồi xuôi

Mồi ngược

287

CMTmC60

ATCAGGCTAAGGCCCAAACT

GCCAATGTAATCTCCCCACA

55

nd

288

CMTm13

AAGCTCCCCAGAAACACAAT

ATTGGGGTCAGAATGAAGGT

46

nd

289

CMTm14

TCTGCTGTCTTCATCTTTGCT

CCAGCAGACAAGCTAATGTGT

55

nd

290

CMTm248

TGATCATGAAAGGGGGAGAG

CCACTTCAAGCTTGCCTTCT

46

nd

291

PU025208

ACAGCAGACTTTGCGAGCTT

AGAGAACCGGAAACCCAAGT

55

nd

292

PU002959

TGAGCAGTCAATATCAACCAAAA

GAGGATTGAAGGCCATGAGA

48

nd

293

comp98739

ATGCACGGTTGCTTGAAACT

CCAAGCAAAATATCCGCCTA

56

nd

294

PU026252

GTCCCTTTGTTGAGCAAGGA

CTTCCAATCGGAAATGGCTA

56

nd

295

PU024278

GGATTTGAGAGCAACCCAGA

CCCCTTTTCCCTCTCTTTTG

60

nd

296

H39

CGTTTTCACAAAACCCTCGT

GAGAAGAGCAACGCTTTCGT

57

nd

297

H41

TAGGTTCAACTCTCTCCCCG

TACTGGTTTTTCCAATCCGC

59

nd

298

H65

ATCATAGTCGTCGTCGGGTC

GCCGATTCTTGAGGAACAGA

55

nd

299

CMTp37

GTCTGGTCTTGGGGTGGTTC

AGAAACAAAGTGGCGGGTGT

53

nd

300

CMTp57

GCCGTCAACACCAAACTCC

AGCGCTGACGGAGGTTAAAT

55

nd


Phụ lục 5. Danh sách 69 chỉ thị SSR sử dụng để phát triển gen bố mẹ và quần thể F2 trong nghiên cứu



TT


Tên chỉ thị

Trình tự mồi


Nhiệt độ gắn mồi

Kích thước sản phẩm

PCR


Mồi xuôi


Mồi ngược

1

CMTp231

TCTGGTCTTGGGGTGGTTCT

AGAAACAAAGTGGCGGGTGT

59

170

2

CMTm48

AAGCCTTTGGGGACCTTTAC

TTGAAACCTTCAAACAAGAAATTG

55

100-120

3

CMTm88

CATCGACATTCGCCTCATC

AGGCAGCTTCCAAATCAGC

54

108-134

4

CMTm188

ACTGAGAGGCCCTGAATTGT

CCCCCTCTTAGTCTTAATATTCTCA

60

158

5

CMTp177

AGCCAAACCAGCATCGAAAT

CGACGATGTCAAACCTCCAC

50

194

6

CMTp224

CACCGACGACTCCATCATC

CTTCTTGTCCCCAAAATCACA

56

155 173

7

CMTp26

GTCTTGGTCTTGGGGTGGTT

AAACAAAGTGGCGGGTGTC

60

187

8

CMTm192

CCAATGCTTTGACAACAACCT

GAAGGAACGGGCATAAGTGA

58

100-120

9

CMTmC62

TGGATCCTCACTCTCAACTCA

GAAGAGTTACTACGTTGCTAGCCTAT

55

140-160

10

CMTp252

TCTCACTCATCCACCACACC

CGTTTCGAGTCATTTGTTCG

47

175 - 208

11

CMTp39

GGCGAAAAGGAAGAACGAAT

TTTTTCTCCCCCTTCCACAT

56

132

12

CMTm10

TGTCTTCGACTGCAAATCCA

CGTCAGGGAGAGGTTTTCAC

55

125

13

CMTm222

GCCGTTTTCTCTCTAGGATGC

TAGGTCCCTGATTCCCTCGT

53

131-139

14

CMTmC61

TTATTGTCGTCGCTCTGCAC

TCCGGAACTATTATAACAACGACA

60

162

15

CMTmC22

TCGAAGTACGTGATGGGACTT

CCATTATGATAAAGCTCCGTTGT

56

155

16

CMTm255

CGCTTCCTTCCTTCTTGGATT

CCACTGTTGTGTAGGGGAAGC

58

91

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Ngày đăng: 11/07/2023