Nghiên cứu ứng dụng chỉ thị phân tử phục vụ chọn tạo giống bí đỏ Cucurbita spp. có hàm lượng chất khô cao - 19



TT


Tên chỉ thị

Trình tự mồi

Nhiệt độ gắn

mồi

Kích thước sản phẩm

PCR

Mồi xuôi

Mồi ngược

35

CMTm54

GTGTGGATGCAAATGGTGAG

GGGAATCGAGGGTTTTGAAT

59

120-141

36

CMTp129

CTCTTGCTCATCTTCCTTGTTG

CCCACCCATTTACCCTCTAGT

54

147

37

CMTp77

GGCCATATACGAGGTCAGGTT

TTAGCGATTCAACGGAGGTC

56

102

38

CMTp62

GTGCCCGTCAGTCGGAAT

TGTCGACGAAGATAGCAATAGCA

57

134

39

CMTp26

GTCTTGGTCTTGGGGTGGTT

AAACAAAGTGGCGGGTGTC

60

187

40

PKCT-47

GGTCCCAATAATAGCAACCAA

GTGGGACACATCTTGAGCA

50

205-269

41

PKCT-111

GTT GCA GCG ACC GTT CTT CTT C

GCA TCT GAA GAC GAT GCG TCG T

56

187-210

42

PKCT-122

CTA AAC AGG ATG CCT CTG ACA C

CGG GAT TTC CGA AAC AAC GT

60

181-225

43

CMTm227

ACTTAGTTGAAAACAACAATTTACCA

TTCTCTCCCAGATTCTAGTTGC

59

209

44

CMTm223

ACAAATCCATGGACATTGAGC

ATTACCGAAGCAAGCACGAT

57

131

45

CMTm29

AGCGCAGCGACAGAAAAG

AGCTTCTACGAAGGCGAGGT

60

91

46

CMTp17

ACTGCTCAATAAGGCAAGGA

AAACAAGAGTGCACAAACAGG

45

70-90

47

CMTm61

GCCATTATTCCACTCCATGC

TGCCTGCACCTGTTTTAGC

52

120-156

48

CMTm192

CCAATGCTTTGACAACAACCT

GAAGGAACGGGCATAAGTGA

58

100-120

49

CMTm232

AGAAAGAAATAAGGAACCCACAG

CGTCTCGCAATTCTTCAACT

59

150-180

50

CMTm239

CAAAGATCTGTTGTGTCAGAGT

GGAGAGTGGAGGAGGTAGAT

57

180-200

51

CMTmC62

TGGATCCTCACTCTCAACTCA

GAAGAGTTACTACGTTGCTAGCCTAT

55

140-160

52

CMTm118

CACTTCACGGACTCCCTTGT

CAGCAGAAGTGCGAAGACAG

56

120-140

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Nghiên cứu ứng dụng chỉ thị phân tử phục vụ chọn tạo giống bí đỏ Cucurbita spp. có hàm lượng chất khô cao - 19



TT


Tên chỉ thị

Trình tự mồi

Nhiệt độ gắn

mồi

Kích thước sản phẩm

PCR

Mồi xuôi

Mồi ngược

53

CMTp249

GCAATCATTAAGGAATGGCACT

CCGACCCATGGATCTTATCTT

55

160-190

54

CMTp252

TCTCACTCATCCACCACACC

CGTTTCGAGTCATTTGTTCG

47

175 - 208

55

CMTm37

CGTGTTTGTGTTGGAAAAGC

AAGCAAACCCACAGAAGGAG

57

13-160

56

CMTm63

GCAGAAGATACCGTCGGAGT

AAGGTGAACGGGAATGTGAG

60

119-139

57

PU000705

AAAGAAAAGCTCGCTTGGTG

TCCATGCCCACATCAACTAA

56

122

58

PU002147

GCTCTGTGAAGGAGGACGAC

CCGCTGCATAGGGAATGTAT

59

239

59

CMTp178

GAACATTTCTGGTTCAATC

CTTTCAAAGAAGCGTAAAG

58

132

60

CMTp202

GTAAGGCGTGGTTGGAAGC

GTTCCCTTTCCCCCTTTGTA

51

156

61

CMTp39

GGCGAAAAGGAAGAACGAAT

TTTTTCTCCCCCTTCCACAT

56

132

62

CMTp229

GATCCTACACGCCGTTTGAT

TCATGACCTCGTCACTCTCG

60

103

63

CMTm10

TGTCTTCGACTGCAAATCCA

CGTCAGGGAGAGGTTTTCAC

55

125

64

CMTm97

AGGGGGAACTGATAGTCATCG

ACCTCACCTTCCATCGTCAC

54

164

65

CMTm222

GCCGTTTTCTCTCTAGGATGC

TAGGTCCCTGATTCCCTCGT

53

131-139

66

CMTp203

GGAATTCCATCTCCGAGTCA

GCATCCTTCCAAAACAAACTTC

55

180-197

67

CMTp21

TGTCCAATTCTTCATTGCTCA

GGATTCCACCACAATTTGAGA

45

201

68

CMTp260

CCCTAGACCCATCATAGTCG

ACATTTGGTTACTTCCCCATT

57

155

69

PKCT-133

TCG GAA TCG TCT TCA GCA ATA GTC

TCC TCT TCC ATT CCA CTT TCT CCT

50

201-251

70

CMTmC61

TTATTGTCGTCGCTCTGCAC

TCCGGAACTATTATAACAACGACA

60

162



TT


Tên chỉ thị

Trình tự mồi

Nhiệt độ gắn

mồi

Kích thước sản phẩm

PCR

Mồi xuôi

Mồi ngược

71

CMTmC52

GTTACGATTTTCATTCTGTCCTG

ACGAATACAAAGTTTTCTCTCTCC

54

151

72

CMTmC37

TCCACACTCAGCTACCACCA

GAAAAGGCAAATGGGGAATC

59

110

73

CMTmC36

TCAAAACGGTAGGAACTTCACA

GCGATGTTTATAGCTTCGGAGT

58

169

74

CMTmC32

CCGCCACCACTGAACAACT

GGCTGTGGCCCTCCATAGT

56

113

75

CMTmC27

AAAGGAACATCAAAATGGTTGC

AGCTTTCAGCTTGCTTTCCA

51

106

76

CMTmC26

TTGAAGCACTGGAAGCCATT

GGAAGGAAGGGGAGAGTGAA

47

173

77

CMTmC22

TCGAAGTACGTGATGGGACTT

CCATTATGATAAAGCTCCGTTGT

56

155

78

CMTm255

CGCTTCCTTCCTTCTTGGATT

CCACTGTTGTGTAGGGGAAGC

58

91

79

CMTm253

GCAACAAATTGTAGTTGCAAAG

GCCAAAGGTTCCAAATGAC

60

155

80

CMTm249

CATCATCAATCGAATAGATTTCATC

GAAAACTCCCTCGTTTACGC

57

164

81

CMTm243

GACAGGACAGGTCAACACCTC

AACCCAATTGCACAGCTTCT

51

139

82

CMTm235

AATCAGGTAGCCAACACCTA

AGACCATTCATAATCAGATCCT

56

107

83

CMTm217

CCCGCTAGCATCACTCATC

TGGGTTCAAAACAAAACGAA

54

92

84

CMTm176

TGCTTTTGGCTTTACCTCTC

CGAGATTTGCATCTTTCGAT

45

128

85

CMTm175

GAGTGATGTTTTGAGTAAACAG

CTTGTTCATCATCATCTGTG

55

94

86

CMTm172

CATTGGAGTGCGTGAAATTG

GTGAGTGGTCAAAGGGCATT

56

115

87

CMTm165

TTCCAGACCCAATTCCCTTA

TCCATGGAGATGGGAGGTAG

59

126

88

CMTp131

GCACTTGAATCTTCGTCAAC

CGAGAAAGAATTAACGAGCA

55

117



TT


Tên chỉ thị

Trình tự mồi

Nhiệt độ gắn

mồi

Kích thước sản phẩm

PCR

Mồi xuôi

Mồi ngược

89

CMTp208

CGTGAACATTCGTTTGTTGG

TCATCCGTTTCCTTTTCAGC

50

120-140

90

CMTmC43

CGAGTAAATCGCCTAACACC

GGGAATTTGTAAATCTTCTCCTC

60

120-130

91

CMTp19

AGGAGGGGAAAATACAGAGT

GCCACTATAAATCCACCACT

55

110-130

92

CMTp32

TCTCTCCAGAGAGTTGCAGGT

AATATCCACCGTCCGATCAT

47

130-140

93

CMTm134

TGCCTTCCTTCCCTGATCTA

ATCAATCAGCTGTGCGTTTG

47

170-180

94

CMTp223

TCCTGTTGAAACCTGAGAAGA

AGGTTTGGGGAATTCAAGAT

60

160-180

95

CMTm87

AATCTGCAAACGGACTTCAAT

TCACAGTGATCCCCAAACTC

56

96-102

96

PU000007

GGAGACCCCATTTTCCTGTT

AAGCACTCACTTCCCCTTCA

58

105

97

PU000308

GGGGGAAGCCATTTATTCTC

CCATGGCCTTACAAGAAGGA

60

194

98

PU000750

AAAGTTGTCCAAAACCGACG

GGCTCGAGAATCTGAAAACG

56

228

99

PU001491

CACAGACGCAGAAACAGAGG

AAGGACAAGATCCATGGGG

45

158

100

PU001551

AAGGAGGAGGAGGTAGCAGC

CCTCGCACTGAGACAACAAA

51

161

101

PU001869

TCCGGTAGAGAATCATTCCG

ATCATCCATCATCGACCCAT

52

177

102

CMTp141

ATTCATGTCATACTACCGACTTC

CGGATTTATAAGATGGCAAG

56

135

103

CMTp53

ACAACCATCACAACGCTTTC

TTGGAATCAGTTCCAGTTCG

60

120

104

CMTp106

ACCCTAAGCCAGATATTCATGC

GGGCTTCATGCATCTAGTTTG

46

153

105

CMTm62

AGTGAGGGAGGCGACAACT

GAACGTGTCCATGTCATTGC

55

100

106

CMTm130

CCCTCCACCACCTCCTTAG

CAGGGGGATCATAAAAGTGG

60

110



TT


Tên chỉ thị

Trình tự mồi

Nhiệt độ gắn

mồi

Kích thước sản phẩm

PCR

Mồi xuôi

Mồi ngược

107

CMTm25

TGAAGCTTTCAGAAATGAATGTG

CTGACGTCGCTACTCATAGCA

60

158

108

CMTp41

TTCTCTCAACCACCGTCACC

GGAGGCCTTGGAATGATAGG

55

141

109

CMTm47

TCCATTCCCAGATTTGAACG

CAGAGCCCACCTAACAGCAG

45

143

110

CMTm155

ACTTGCGGACTTTCACACCT

TGAAATGAACGCAGAATTGC

55

137-144

111

CMTm247

CCAAAATTCACTAGAAGTAACGATCA

ATGCAACGAAGTTGGGGTAG

56

120-137

112

CMTp144

ATGGCTTCCAAGCTCCTCTT

GTCGGCCATGAGCTTGAG

45

103-118

113

CMTp121

CCCAACCCGTCTCATAAACA

TTTAGGCCCAATGTTCTTGCT

59

217

114

CMTp239

ATCTCGGTAAAGCTCCGTTC

TATGGTTCTGCCTCCACAAT

56

101

115

CMTp264

TGTCCATAGTCGAACGTCCA

CCCATTTGTGATGATGAGTACG

60

104

116

PKCT-62

GAA GTT CGT GGT CTG TGC AAG TC

CCT GAG TAA CCT CCG TGC TTC C

60

300-326

117

CMTmC70

CAGGCTATTCGCACCCTCTA

CCTCATGCATTTTGCGTGTA

58

196

118

CMTmC69

AGGCAGTGATATAACCATGAAATG

TCATAACATAACATATCGCATCACA

56

188

119

CMTmC63

CAAATGAAAGAGTTTAAGCTTCAGTG

ATGCCTGCATTTCCAGAGTT

57

91

120

CMTmC56

GGGCTCTCTCAACCTCACTC

CCGGGAATGAAACAAACAC

60

179

121

CMTmC55

GTCATCTCATCATCATGCATCT

AATGTTATATGATGTTACACGATGG

54

113

122

CMTmC53

GAGGCGAAAATGATCAAACAG

CATCACGTTCATTGGCATTC

56

196

123

CMTmC51

CCTCAACAAATTCCTCCATTG

CTCCAATATCACCGAGAGTCG

45

118

124

CMTmC46

TATGAAGTGTCGTCGGTTCG

CAGTGTTTGATGCAGCCATT

59

134



TT


Tên chỉ thị

Trình tự mồi

Nhiệt độ gắn

mồi

Kích thước sản phẩm

PCR

Mồi xuôi

Mồi ngược

125

CMTmC44

ACCTTCCTCCTCCGTAATGT

GACGAAGCCAACAACTACAAC

55

112

126

CMTmC42

TCACAAAGTCAAAACAGACAAACA

TGTTTCAGGGAAATGAAGAGG

56

105

127

CMTmC41

TCCCTTCCAACCAAGTAAGC

CCTAACCTGGTATTGTTTATGATCC

47

107

128

CMTmC40

CTCCTACGTAAGGGCTGTGTG

GCAATGAAATGATGGGACCT

55

135

129

CMTmC38

TGTTCATAATATTACATTTTCTTCGTC

AATTAGGGATTGAATTGAAATGAT

59

153

130

CMTmC35

CACGCCTCTGATTCTCAACA

GTTTCCATTGATGGGGTTGT

60

109

131

CMTmC33

TCGTTGTTGTAGCCTTTTTGC

AAGCGAGGAAGAGAGGTTTTG

56

105

132

CMTmC31

GGGCAACACCACAAATGAAC

GATGCCTCCACCATCGAGTA

50

113

133

CMTmC28

GGTCAAATTCAAGGGCTTACC

AGGAGCATCCTTGTCGTTGT

58

111

134

CMTmC20

GAGACGTTTGGCTTTTCTTG

AAGAAGTTCCTCGATGGTCA

56

176

135

CMTmC17

CCCGTGGGATATCATTAGATAAG

AATATGTCCCTGCCTGGGTA

57

100

136

CMTmC16

TTATAAGAATGATGTTACTCGAT

CATCATCATACATCTTACATTG

60

105

137

CMTmC11

TTGCAACATTTGACTAAATTCCA

CTCCTCTTCTGCCGATCAGT

56

127

138

CMTmC10

CCCTTACATTCGTCCCTTGT

AATATTGGGCTTCCATACGC

60

121

139

CMTmC6

CACAGATGCCTACTGAAAATGC

TGATCGGCGAGAAGGTATG

60

109

140

CMTmC5

GGACCAAAATCAAACCAGAAACT

CGGGAAATAATTCGGGTTCT

51

93

141

CMTmC3

CATCAGGGGTACTTCCCATT

ACTTCCGGAGGTCAGAAGAC

53

131

142

CMTm260

ACAATGGTTCTTTTGATCCTTGA

TGCAAACAATGTGTGTGTGTG

56

90



TT


Tên chỉ thị

Trình tự mồi

Nhiệt độ gắn

mồi

Kích thước sản phẩm

PCR

Mồi xuôi

Mồi ngược

143

CMTm250

GCCGCTCTATAAAGTACCTCTCTC

GCGATCCCATTCTCAGTTCT

57

138

144

CMTm244

GTAAAAGAGAAGGAATACGG

CAGCTACAAAATAGTTACTGAAC

56

120

145

CMTm240

ACCATTGCCATTTCTGAAGG

TTTGAGTGGGTGCTTGTCAG

59

150

146

CMTm236

TTGTTGACTCTGGCAAATCG

TCGAGTCTTCAGCATTATCAGC

56

121

147

CMTm234

GGTGTCGGAAACGAGATAAGG

CATAAGGGCGCTCAATTTCA

47

193

148

CMTm220

AAGCCAAAGCCCCTTATCT

TTTTTGAAATTCTTTGCATCACT

55

133

149

CMTm212

AACCTAACCCCCAAAACAGC

AAATGCGCAGAAGAATCCAG

60

118

150

CMTm204

GGTCATAATATTTTCAACCCCATC

TTCTCTTACACCAATTTTGTTTGG

50

154

151

CMTm203

GATACCGTTTTTCGAATCAAATC

CAAATTGATAGGAATGTGCAGAG

55

102

152

CMTm202

GGTGCTCAATTACCCCTGCT

CAACCTGACAGGCAAACCAG

60

108

153

CMTm199

GGACACGTATCCGTCACCTT

TTCCCCATCTGCATGTTACTC

55

192

154

CMTm196

TCACGAAAAACCCTAAACC

GTTAACCCAAATCAAAATGGT

58

128

155

CMTm189

AATTATATAGCTGGCTGGATCG

AACATACAACAGCAGGGAATG

56

106

156

CMTm182

AGTTGGCTTTAGCACATGG

GGACATCAGAAGCCACAGT

59

111

157

CMTm164

AGCCTTTCAGAAGAACCAAG

GGCTTCAAACAAATACTAACCA

51

140

158

CMTm162

TGGATGGGGAAATTATTGGA

ATTCAGGGGAGATTGGGACT

56

97

159

PU000104

TCGGATCCACTCCCATAGAC

AAACCATGCCTCTGACAACC

54

233

160

PU000716

TCCGGTAGAGAATCATTCCG

ATCATCCATCATCGACCCAT

58

189



TT


Tên chỉ thị

Trình tự mồi

Nhiệt độ gắn

mồi

Kích thước sản phẩm

PCR

Mồi xuôi

Mồi ngược

161

PU001527

CAACTGCTACACCAAGCCAA

GGAGGTGGGGGTAGAGAGAC

46

160

162

PU001748

TGCAATCCATAATTCATAATT TAACC

CACACGACGCTGTGAAAGAT

56

188

163

PU001821

CGGCAGTGATGACTGAGAAA

CGCTACCATGTGGACAGAGA

60

204

164

PU002054

CAACTGCTACACCAAGCCAA

GGAGGTGGGGGTAGAGAGAC

59

164

165

PU002121

ATCGATAAGAAAGACGGCGA

CCTTCAGGTCCGAGTTTCAA

56

199

166

PU002158

GTCGGGGTAGGGAGAAACTC

AGTCGGAACAACGAATCCAC

59

251

167

PU002184

GGGGATGCAAAAAGTGAAAA

CCCCAACAAAATCCCTTCTT

56

124

168

PU002200

GGGCTTCGAGGACATATTGA

GCCGAAAAGCTTTCTCACAC

57

219

169

CMTp84

GCGGTAACAGGTTGTTTGGT

CCATCAGGATACCCTACAAAGG

45

150

170

CMTm8

AGACTAAGTTTTCAGGCAGT

GAACTTGTAAATGATCTATGTTG

55

137-148

171

CMTm12

CATGTTTTCTCATTCACAAGCTG

AGGGCGTTGTTAGGTTTGTG

60

115-123

172

CMTm39

ATCTTTGTGCTTCTAACCTTTTTC

TAAAGCTCGAAAAAGCAACC

57

144-160

173

CMTm96

CGTCCATTTGGAAGCTCAGT

CATCCTCGTTCCTTGAGATCAT

55

101-111

174

CMTm157

GGACAGAGTGGCTAGGTCTGAGT

CCAAACAAGAGATGGGCAGA

59

123-129

175

CMTm177

CAGACGTCGAACGTTGTCAC

CTGGAGAGGAGCATTTGGAG

56

101-115

176

CMTm186

TCCAAATTTCAGGGAAGTGAGT

CCGAGATGAAGAAGGACGAG

56

139-147

177

CMTm190

GCATTGGATAACCTTATTCTG

CTCCTTCGACAGGGAAG

58

102-114

178

CMTm214

TGCTTTGATCAGTGAGCAGT

GGATGTACTCTCCGGACTTTC

47

130-142

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Ngày đăng: 11/07/2023