Nghiên cứu phát triển chủng nấm sợi và tối ưu điều kiện lên men sản xuất đa enzyme α-amylase, glucoamylase, cellulase ứng dụng trong chế biến thức ăn chăn nuôi - 18

Dependent Variable: AMY


Source

DF

Sum of Squares

Mean Square

F Value

Pr > F

Model

4

2.53820000

0.63455000

0.35

0.8411

Error

10

18.34693333

1.83469333



Corrected Total

14

20.88513333




R-Square

Coeff Var

Root MSE

AMY Mean



0.121531

3.525836

1.354509

38.41667



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Nghiên cứu phát triển chủng nấm sợi và tối ưu điều kiện lên men sản xuất đa enzyme α-amylase, glucoamylase, cellulase ứng dụng trong chế biến thức ăn chăn nuôi - 18

Dependent Variable: CEL


Source

DF

Sum of Squares

Mean Square

F Value

Pr > F

Model

4

1.35610667

0.33902667

0.21

0.9254

Error

10

15.93526667

1.59352667



Corrected Total

14

17.29137333




R-Square

Coeff Var

Root MSE

CEL Mean



0.078427

10.09503

1.262350

12.50467




3. Tối ưu điều kiện lên men xốp

3.1. Cơ chất

3

3

3

7.0796262

7.0463438

12.5395217

3

3

3

7.6484394

7.6097323

2.6466999

3

3

3

8.2321611

8.1696922

13.9280954

3

3

3

7.7580540

7.6968951

3.9995970

The MEANS Procedure


COCHAT

CAMGAO

N Obs

3

Variable


GLU

N

Mean


27.0066667

Std Error


1.1038770

Std Dev Coeff of Variation


1.9119711



AMY


38.7700000

1.5772444

2.7318675



CEL


12.2033333

0.8834843

1.5302396

CAMMI

3

GLU


34.7833333

1.5359724

2.6603822



AMY


49.8800000

2.1914683

3.7957344



CEL


16.7400000

0.2557994

0.4430576

MUNCUA

3

GLU


17.4700000

0.8303212

1.4381585



AMY


25.1500000

1.1862687

2.0546776



CEL


9.8066667

0.7885923

1.3658819

TRAU

3

GLU


13.3366667

0.5973646

1.0346658



AMY


19.2466667

0.8552842

1.4813957



CEL


6.3400000

0.1464013

0.2535744

The ANOVA Procedure

Dependent Variable: GLU


Source

DF

Sum of Squares

Mean Square

F Value

Pr > F

Model

3

836.3166917

278.7722306

80.38

<.0001

Error

8

27.7442000

3.4680250



Corrected Total

11

864.0608917




Dependent Variable: AMY


Source

DF

Sum of Squares

Mean Square

F Value

Pr > F

Model

3

1706.190300

568.730100

80.42

<.0001

Error

8

56.573867

7.071733



Corrected Total

11

1762.764167




Dependent Variable: CEL


Source

DF

Sum of Squares

Mean Square

F Value

Pr > F

Model

3

171.7146917

57.2382306

51.24

<.0001

Error

8

8.9357333

1.1169667



Corrected Total

11

180.6504250





Tukey's Studentized Range (HSD) Test for GLU


Tukey Grouping

Mean

N

COCHAT

A

34.783

3

CAMMI

B

27.007

3

CAMGAO

C

17.470

3

MUNCUA

C

13.337

3

TRAU

Tukey's Studentized Range (HSD) Test for AMY


Tukey Grouping

Mean

N

COCHAT

A

49.880

3

CAMMI

B

38.770

3

CAMGAO

C

25.150

3

MUNCUA

C

19.247

3

TRAU

Tukey's Studentized Range (HSD) Test for CEL


Tukey Grouping

Mean

N

COCHAT

A

16.7400

3

CAMMI

B

12.2033

3

CAMGAO

B

9.8067

3

MUNCUA

C

6.3400

3

TRAU

3.2. Độ ẩm

3

3

3

8.7951459

8.7965213

21.0701740

3

3

3

3.0996333

2.0008675

20.7709268

3

3

3

7.7736146

4.4902470

3.5706370

The MEANS Procedure


DOAM

20

N Obs

3

Variable


GLU

N

Mean


16.8433333

Std Error


0.8552842

Std Dev


1.4813957

Coeff of Variation



AMY


21.8200000

1.1081667

1.9194009




CEL


10.6633333

1.2971807

2.2467829


30

3

GLU


33.9900000

0.6082763

1.0535654




AMY


45.5333333

0.5260017

0.9110617




CEL


16.5866667

1.9890897

3.4452044


40

3

GLU


34.7100000

1.5578190

2.6982216




AMY


46.4233333

1.2034995

2.0845223




CEL


17.7333333

0.3655741

0.6331930


50

3

GLU


39.1600000

0.7892401

1.3670040




AMY


52.6433333

0.2556256

0.4427565




CEL


22.7466667

0.6933333

1.2008886


60

3

GLU


22.8633333

1.8767022

3.2505435




AMY


30.0433333

2.2162005

3.8385718




CEL


10.1566667

0.9404491

1.6289056


70

3

GLU


15.5066667

1.1715564

2.0291952




AMY


19.9466667

1.6133230

2.7943574




CEL


7.0166667

0.8676661

1.5028418



3

3

3

3.4908172

0.8410495

5.2794046

3

3

3

14.2172775

12.7767839

16.0377968

3

3

3

13.0859538

14.0091448

21.4181722

The ANOVA Procedure

Dependent Variable: GLU


Source

DF

Sum of Squares

Mean Square

F Value

Pr > F

Model

5

1525.033178

305.006636

67.44

<.0001

Error

12

54.274600

4.522883



Corrected Total

17

1579.307778




Dependent Variable: AMY


Source

DF

Sum of Squares

Mean Square

F Value

Pr > F

Model

5

2912.299917

582.459983

110.60

<.0001

Error

12

63.196933

5.266411



Corrected Total

17

2975.496850




Dependent Variable: CEL


Source

DF

Sum of Squares

Mean Square

F Value

Pr > F

Model

5

515.0048944

103.0009789

26.11

<.0001

Error

12

47.3448000

3.9454000



Corrected Total

17

562.3496944





Tukey's Studentized Range (HSD) Test for GLU


Tukey Grouping

Mean

N

DOAM

A

39.160

3

50

A

34.710

3

40

A

33.990

3

30

B

22.863

3

60

C

16.843

3

20

C

15.507

3

70

Tukey's Studentized Range (HSD) Test for AMY


Tukey Grouping

Mean

N

DOAM


A

52.643

3

50

B

A

46.423

3

40

B


45.533

3

30


C

30.043

3

60


D

21.820

3

20


D

19.947

3

70

Tukey's Studentized Range (HSD) Test for CEL


Tukey Grouping

Mean

N

DOAM


A

22.747

3

50

B

A

17.733

3

40

B


16.587

3

30


C

10.663

3

20


C

10.157

3

60


C

7.017

3

70

3.3. Nhiệt độ

3

3

3

11.4264775

11.7461925

17.1667712

3

3

3

13.5707032

13.7715920

22.7798332

3

3

3

4.7736973

4.8157466

1.6028360

The MEANS Procedure


ND N Obs

20C 3

Variable


GLU

N

Mean


14.7700000

Std Error


0.9743887

Std Dev


1.6876907

Coeff of Variation


AMY


20.5300000

1.3922763

2.4114933



CEL


6.0133333

0.5959959

1.0322952


25C 3

GLU


27.6633333

2.1674358

3.7541089



AMY


38.9400000

3.0961320

5.3626579



CEL


13.0600000

1.7176437

2.9750462


30C 3

GLU


39.7700000

1.0960991

1.8984994



AMY


56.2400000

1.5636816

2.7083759



CEL


22.7700000

0.2107131

0.3649658


35C 3

GLU


24.2366667

1.6788521

2.9078572



AMY


34.0500000

2.3995069

4.1560679



CEL


10.5266667

0.3307735

0.5729165


40C 3

GLU


13.2166667

0.5795496

1.0038094



AMY


18.3000000

0.8268212

1.4320964



CEL


10.6600000

0.9357528

1.6207714


45C 3

GLU


7.3266667

1.1489464

1.9900335



AMY


9.8900000

1.6381697

2.8373932



CEL


4.5500000

0.3251666

0.5632051


3

3

3

11.9977603

12.2057793

5.4425255

3

3

3

7.5950271

7.8256632

15.2042347

3

3

3

27.1615127

28.6895164

12.3781344

The ANOVA Procedure

Dependent Variable: GLU


Source

DF

Sum of Squares

Mean Square

F Value

Pr > F

Model

5

2080.142961

416.028592

73.48

<.0001

Error

12

67.938867

5.661572



Corrected Total

17

2148.081828




Dependent Variable: AMY


Source

DF

Sum of Squares

Mean Square

F Value

Pr > F

Model

5

4245.438850

849.087770

73.53

<.0001

Error

12

138.566600

11.547217



Corrected Total

17

4384.005450




Dependent Variable: CEL


Source

DF

Sum of Squares

Mean Square

F Value

Pr > F

Model

5

627.5082667

125.5016533

56.52

<.0001

Error

12

26.6441333

2.2203444



Corrected Total

17

654.1524000





Tukey's Studentized Range (HSD) Test for GLU


Tukey Grouping

Mean

N

ND


A

39.770

3

30C


B

27.663

3

25C


B

24.237

3

35C


C

14.770

3

20C

D

C

13.217

3

40C

D


7.327

3

45C

Tukey's Studentized Range (HSD) Test for AMY


Tukey Grouping

Mean

N

ND


A

56.240

3

30C


B

38.940

3

25C


B

34.050

3

35C


C

20.530

3

20C

D

C

18.300

3

40C

D


9.890

3

45C

Tukey's Studentized Range (HSD) Test for CEL


Tukey Grouping

Mean

N

ND

A

22.770

3

30C

B

13.060

3

25C

B

10.660

3

40C

B

10.527

3

35C

C

6.013

3

20C

C

4.550

3

45C

3.4. pH môi trường

3

3

3

9.3561855

10.3312883

13.2579454

3

3

3

9.8266947

10.6545422

15.8634995

3

3

3

8.0967358

8.6901413

7.9712424

3

3

3

5.9439323

6.2846141

9.0007704

3

3

3

1.5389949

1.5854929

7.3899363

3

3

3

5.7542393

6.3063661

5.7120545

3

3

3

5.3607144

5.6293750

16.0290210

3

3

3

7.8766416

8.4707777

8.7345384

3

3

3

3.8243150

4.2696097

22.6579762

The MEANS Procedure


PH

PH3

N Obs

3

Variable


GLU

N

Mean


15.7566667

Std Error


0.8511430

Std Dev


1.4742230

Coeff of Variation



AMY


20.3800000

1.2156206

2.1055166




CEL


11.7600000

0.9001667

1.5591344


PH35

3

GLU


18.8733333

1.0707682

1.8546248




AMY


24.8233333

1.5269832

2.6448125




CEL


12.5166667

1.1463759

1.9855814


PH4

3

GLU


21.4500000

1.0027130

1.7367498




AMY


28.5100000

1.4304195

2.4775593




CEL


12.8500000

0.5913826

1.0243046


PH45

3

GLU


27.8500000

0.9557371

1.6553852




AMY


37.6500000

1.3661015

2.3661572




CEL


14.7833333

0.7682303

1.3306139


PH5

3

GLU


37.2266667

0.3307735

0.5729165




AMY


51.0466667

0.4672734

0.8093413




CEL


21.6266667

0.9227194

1.5981969


PH55

3

GLU


42.4566667

1.4105003

2.4430582




AMY


55.3166667

2.0140700

3.4884715




CEL


22.9600000

0.7571878

1.3114877


PH6

3

GLU


30.5400000

0.9452160

1.6371622




AMY


41.4933333

1.3485836

2.3358153




CEL


16.2033333

1.4995147

2.5972357


PH65

3

GLU


21.0700000

0.9581753

1.6596084




AMY


27.9666667

1.3677394

2.3689942




CEL


10.3833333

0.5236199

0.9069362


PH7

3

GLU


14.7833333

0.3264115

0.5653612




AMY


18.9833333

0.4679506

0.8105142




CEL


8.0900000

1.0583005

1.8330303


The ANOVA Procedure


Dependent Variable: GLU


Source

DF

Sum of Squares

Mean Square

F Value

Pr > F

Model

8

2236.975296

279.621912

107.45

<.0001

Error

18

46.841133

2.602285



Corrected Total

26

2283.816430




Dependent Variable: AMY


Source

DF

Sum of Squares

Mean Square

F Value

Pr > F

Model

8

4128.646667

516.080833

97.34

<.0001

Error

18

95.429600

5.301644



Corrected Total

26

4224.076267




Dependent Variable: CEL


Source

DF

Sum of Squares

Mean Square

F Value

Pr > F

Model

8

592.4729407

74.0591176

27.32

<.0001

Error

18

48.7911333

2.7106185



Corrected Total

26

641.2640741





Tukey's Studentized Range (HSD) Test for GLU


Tukey Grouping

Mean

N

PH


A

42.457

3

PH55


B

37.227

3

PH5


C

30.540

3

PH6


C

27.850

3

PH45


D

21.450

3

PH4


D

21.070

3

PH65

E

D

18.873

3

PH35

E


15.757

3

PH3

E


14.783

3

PH7


Tukey's Studentized Range (HSD) Test for AMY


Tukey Grouping

Mean

N

PH

A

55.317

3

PH55

A

51.047

3

PH5

B

41.493

3

PH6

B

37.650

3

PH45

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