Panel 4.14: Dendrobium orchid shoots after 60 days of in vitro cultureon medium supplemented with TDZ and NAA
Table 4.8: Effects of TDZ and NAA on shoot growth and development
Dendrupium in vitro culture after 90 days.
Solution | Number of shoots / | Number of leaves | Number of roots | Height | |
VISS MS + 0.05mg/l TDZ MS + o.lmg/l TDZ MS + 0.5mg/l TDZ MS Wallet + lmg/1 TDZ Vi 2.00 B MS+0.05mg/lT Vx MS+0.1 mg/lTDZ+0.5mg/lNAA v% MS+0.5mg/lTDZ+0.5mg/lNAA MS+1mg/lTDZ+0.5mg/lNAA | 3.33 A 5.78B 7.56 CD 8.89 DEF 9.44 F 1.36° 7.89 CDE 9.22 ^ 10.22 p | 2.17 B 2.16 B 1.90B 2.06 B 1.71 ^ 2.08 B 1.79 B 1.23 A | 00 | 1.31 ® 1.11 BC 0.56 A 0.42 A 1.05 B 0.49 A 0.48 A | 2.39 H 2.04 DE 1.67 CD 1.27" 1.05 A 1.93 00 1.64 80 1.12 A 1.02 A |
cv% | 3.33% | 1.81% | 1.55% | 1.61% | |
-------------------------------------------------- -------------------------------------------------- -------------------------------------------------- -------------------------------------------------- -------------------------------------------------- ------------------------------------------------¥- ---------------------------------------- *Values followed by letters in the same column with different characters indicate | |||||
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The difference is statistically significant at the 0.05 level.
Comment:
■
Through the statistical classification of the experimental results above, it shows that
After 90 days of culture in vitro , the following symptoms were observed:
*number of buds:
Based on table 4.8, it shows that there are statistically significant differences between the culture environment factors.
At the 90-day culture stage, the number of shoots increased in the media supplemented with high TDZ concentrations and even higher when we combined TDZ and NAA:
- In MS + 0.5mg/l TDZ medium , number of shoots: 8.89.
- In Vz MS medium + 0.5mg/l TDZ + 0.5mg/l NAA, number of shoots: 9.22.
- In MS Vi medium + lmg/l TDZ number of shoots: 9.44.
- In the medium l Á MS + 1mg/1 TDZ + 0.5mg/l NAA, the number of shoots: 10.22.
Thus, at the stage after 90 days of culture, TDZ and NAA grass greatly affected shoot formation.
*number of leaves, roots and shoot height:
- Number of leaves: Table 4.8 shows that the number of leaves did not increase in environments supplemented with high levels of TDZ and TDZ combined with NAA. This proves that TDZ and TDZ combined with NAA do not affect the leaf formation process.
- Number of roots: Table 4.8 also shows that the number of roots decreased while the number of shoots increased.
- Shoot height: shoot height is often inversely proportional to shoot formation, so in environments with high shoot formation, shoot height decreases.
Figure 4.16: Dendrobium orchid shoots after 90 days of in vitro culture on medium supplemented with TDZ and NAA.
4.3. General comments
43.1. Comparison between in vitro culture media on the formation of somatic embryos, protocorms and shoots in two orchid species Dendrobium and Cymbidium * For Cymbidium orchid wells
Chart 4.1: Comparison of the ability to form somatic embryos, protocorms and buds between the environments
Cymbidium orchid culture
* For Dendrobium orchids
BA=10mgrt BA=10mg/HNAA=0.5mgrt TDZ=1mgfl TDZ=1mg/l+NAA=0.6mgJl
ENVIRONMENT
Figure 4.2: Comparison of the ability to form somatic embryos, protocorms and shoots between culture media on Dendrobium orchid species
Based on charts 4.1 and 4.2 shows:
- 1/2 MS + lmg/1 TDZ + 0.5mg/l NAA medium showed the highest number of somatic embryos, protocorms and shoots.
- Media supplemented with NAA showed higher ability to form somatic embryos, protocorms, and shoots than media without NAA supplementation.
Thus, in the in vitro propagation process , the medium of 1/2 MS + 1 mg/1 TDZ + 5 mg/l NAA showed high in vitro propagation ability on two orchid varieties Dendrobium and Cymbidium .
4.3.2» Comparison of the ability to form somatic embryos, protocorms and buds between the two orchid varieties Dendrobium and Cymbidìum
* Comparison of somatic embryogenesis ability between 2 orchid wells
ENVIRONMENT
Chart 43: Comparison of somatic embryogenesis ability between two orchid varieties
r
Dendrobium and Cymbidium after 60 days of growing plants
Table 4.4: Comparison of protocorm formation ability between 2 orchid wells
Dendrobium and Cymbidium after 60 days of in vitro culture .
Chart 4.5: Comparison of bud formation ability between 2 orchid varieties
Dendrobium and Cymbidium after 90 days of in vitro culture
* Compare the height of 2 orchid varieties after 90 days of in vitro culture
2.5 Yes
Table 4.6: Comparison of height growth between 2 orchid varieties
Cymbidium after 90 days of in vitro culture
Dendrobium and
*Comment:
Based on the above charts, it shows that between the two orchid varieties,
Dendrobium has the ability to
The ability to form somatic embryos, protocorms and buds is much higher than that of Cymbidium.
Although the ability to form somatic embryos, protocorms and shoots is higher, the height development of shoots is lower than Cymbidium.