(NSLT) is the ability to give the highest yield of a variety under optimal conditions and shows the yield potential of the variety. In fact, NSLT and actual yield are far apart. NSLT of pumpkin depends on factors such as planting density, number of fruits/plant, average fruit weight. There are many different factors that determine the number of fruits/plant, in addition to the variety factor, it is also greatly affected by external conditions and care techniques. However, if under the same care conditions and the same care techniques, the number of fruits/plant is determined by the variety factor. The pumpkin variety that flowers more or less, the rate of flowering, fruit set, long or short growth period all affect the number of fruits/plant. Therefore, when the plant density is the same, NSLT depends on the average number of fruits/plant and the average fruit weight. Varieties with a large number of fruits and a large fruit weight are varieties with high yield potential. The results of evaluation and grouping of pumpkin varieties according to yield components are presented in Table 3.3.
Table 3.3. Grouping of studied pumpkin varieties according to yield components
Traits
Range of oscillation | Expression state | |||
Variation values of the samples (subgroups) | Number of samples | Rate (%) | ||
Number of fruits/tree (fruit) | Minimum: 0.40 Maximum: 2.50 Average: 1.23 | Low (< 1.0) | 35 | 26.51 |
Average (1.0 -2.0) | 92 | 69.70 | ||
Multiple (>2.0) | 5 | 3.79 | ||
Mass fruit (kg) | Minimum: 0.7 Largest: 5.3 Average: 2.1 | Small (<2) | 102 | 77.27 |
Average (2 -5) | 27 | 20.45 | ||
Large (> 5) | 3 | 2.27 | ||
NSLT (ton/ha) | Minimum: 8.9 Largest: 19.9 Average: 15.07 | Low (< 10 ) | 7 | 5.30 |
Medium (10-15) | 56 | 42.43 | ||
High (> 15 ) | 69 | 52.27 | ||
NSTT (ton/ha) | Smallest:7.7 Largest:17.7 Average:12.66 | Low (< 10 ) | 28 | 22.72 |
Medium (10-15) | 88 | 65.16 | ||
High (> 15 ) | 16 | 12.88 | ||
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Number of Samples of Each Group of Varieties Originating from Collection Sub-Areas and Rubber Tree Genebank Conservation Centers
The number of fruits per plant of pumpkin varieties ranged from 0.4 - 2.5 fruits/plant, with an average of 1.23 fruits/plant. There were 35 varieties with low fruit number (<1 fruit) accounting for 26.51%, 92 varieties (69.7%) with fruit number ranging from 1-2 fruits and the remaining 5 varieties with high fruit number (>2 fruits) accounting for 3.79%.
Fruit weight determines the productivity of the tree. Fruit weight is often prioritized by breeders for research and selection towards small to medium fruit weight, suitable for a family's meal (Nguyen Thi Tam Phuc et al. (2017 ) [14], Tran Danh Suu and Nguyen Thi Tam Phuc (2018) [15]).
According to Gerardus & Grubben (2004) [48], the weight of a normal pumpkin ranges from 0.5 kg to around 10 kg. The results of the group assessment show that the weight of the pumpkin varieties in the group fluctuates quite a lot, ranging from 0.7 - 5.3 kg. The variety with the largest average weight is the Nhung Nghim SDK 7528 variety, the largest average weight is the Nhung Nghim SDK 7528 variety, collected in Bac Kan (5.3 kg), the smallest is the Tau Dau SDK 19273 variety collected in Lao Cai (0.7 kg). Of which, the weight of the small fruit form (<2kg) has 102 varieties, accounting for 77.27%; the medium fruit form (2-5kg) has 27 varieties, accounting for 20.45%; the smallest is the large fruit form (>5kg) with 3 varieties, accounting for 2.27%. The pumpkin varieties of the research group mainly have small to medium weight, which can be explained by the fact that small fruit weight is often associated with better fruit quality than large fruit weight. Moreover, in most midland and mountainous provinces, pumpkin production is mainly self-sufficient, so pumpkin varieties with medium and small weight are selected for the purpose of making vegetables, suitable for daily meals. According to the research results of Ha Minh Loan, (2020) [11], local pumpkin varieties fluctuate quite a lot from 0.59 - 4.96 kg. Most pumpkin varieties in this study have small to medium fruit weight.
Theoretical yield (TH) of pumpkin varieties ranged from 8.9-19.9 tons/ha, averaging 15.07 tons/ha. There were 7 varieties with low TH (<10 tons/ha) accounting for 5.3%, 56 varieties with average TH (10-15 tons/ha) accounting for 42.43% and the remaining 69 varieties with high TH (>15 tons/ha).
Actual yield ( AY) is a synthetic factor that evaluates the entire growth, development, and production efficiency of pumpkin plants. Experimental results show that the AY of the varieties fluctuates from 7.7 to 17.7 tons/ha; the Ca Deng Nu variety (SDK 5354) has the highest yield of 17.7 tons/ha. There are 88 varieties with average AY (10-15 tons/ha), accounting for 65.16%, 16 varieties with high AY (>15 tons/ha), accounting for 12.88%, and the remaining 28 varieties with low AY (<10 tons/ha), accounting for 22.72%.
3.1.3. Evaluation of some quality indicators of pumpkin varieties
Fruit quality is a characteristic of pumpkin varieties determined by genes and is less affected by external conditions and is an important trait for classifying varieties. In pumpkin, quality components such as: Brix, dry matter content, vitamin C, -carotene vary and depend on the time of harvest as well as the purpose of use, consistent with the study of Sonu Sharma and Ramana Rao, (2013) [106].
The results of quality assessment of 132 pumpkin varieties through analysis of some quality indicators in the laboratory such as brix, dry matter content, vitamin C, -carotene are presented in Table 3.4.
The data in Table 3.4 shows:
- Brix (%) is a quality indicator that shows sweetness, playing an important role in assessing the quality of fruits and vegetables. One Brix degree corresponds to 1g of sucrose in 100g of solution. Published studies by Nguyen Thi Tam Phuc et al. (2017) [14] determined that for pumpkin, a brix degree above 10 is good. Research results showed that the measured brix degree of 132 pumpkin varieties ranged from 3.1 - 12.3%; in which the majority of varieties (118 samples, 89.4%) had a brix degree <10%; Only 14 varieties (accounting for 10.6%) have a brix level higher than 10%, including varieties with registration numbers ( SĐK ): 5352, 5363, 6551, 6552, 6559, 6561, 6741,
6740, 6916, 8382, 8396, 8576, 15113 and 19300; these are potential varieties.
quality energy, tastier and more nutritious for users and will be a valuable source of material for quality breeding.
- Brix (%) is a quality indicator that shows sweetness, playing an important role in assessing the quality of fruits and vegetables. One Brix degree corresponds to 1g of sucrose in 100g of solution. Published studies by Nguyen Thi Tam Phuc et al. (2017) [14] determined that for pumpkin, a brix degree above 10 is good. Research results showed that the measured brix degree of 132 pumpkin varieties ranged from 3.1 - 12.3%; in which the majority of varieties (118 samples, 89.4%) had a brix degree <10%; Only 14 varieties (accounting for 10.6%) have a brix level higher than 10%, including varieties with registration numbers ( SĐK ): 5352, 5363, 6551, 6552, 6559, 6561, 6741,
6740, 6916, 8382, 8396, 8576, 15113 and 19300; these are potential varieties.
quality energy, tastier and more nutritious for users and will be a valuable source of material for quality breeding.
Table 3.4. Some quality indicators of studied pumpkin varieties
Nutritional composition
Range of oscillation | Range of variation | Number of samples | Rate (%) | |
Brix (%) | Minimum: 3.1 Largest: 12.3 TB: 7.6 | ≤ 10% | 118 | 89.4 |
> 10% | 14 | 10.6 | ||
Dry matter content (%) | Smallest: 2.2 Largest: 19.0 Average: 7.9 | < 6% | 29 | 38.28 |
6 - 10% | 82 | 62.12 | ||
> 10% | 21 | 15.9 | ||
β-carotene content (µg/g) | Largest: 23.6 Minimum: 2.8 Average: 7.6 | < 5.0 µg/g | 31 | 23.5 |
5.0 - 10.0 µg/g | 86 | 65.2 | ||
10.0 - 15.0 µg/g | 13 | 9.8 | ||
> 15.0 µg/g | 2 | 1.5 | ||
Vitamin C content (mg/100g) | Minimum: 1.4 Largest: 19.1 Average: 6.3 | < 5 mg/100g | 53 | 40.1 |
5 - 10 mg/100g | 67 | 50.8 | ||
10 - 15 mg/100g | 10 | 7.6 | ||
>15 mg/100g | 2 | 1.5 |
Note: Dry matter content (%) is classified according to TCVN 10696:2015 [20]; Classifications are based on the classification of the Nutrient Data Laboratory, United States Department of Agriculture (2006) [121].
Under the same growing conditions, the quality of the varieties is determined by the variety factor. Different varieties have different qualities. Pumpkin is a vegetable with high nutritional value, good for health, the higher the dry matter content in pumpkin, the greater the nutritional value in pumpkin. Dry matter content is the remaining content of pumpkin after the water has been removed during the drying process. The results of the dry matter content assessment of the research varieties are shown in Table
3.4 and Figure 3.11.
The evaluation results showed that the dry matter content of the studied pumpkin varieties had large differences, ranging from 2.2% to 19.0%. with an average value of 7.9%, a standard deviation of 2.6 and a coefficient of variation of 33.4%. The variety with the lowest dry matter content of 2.2% was Co Nhum (SDK-19326, collected in Bac Kan), with a yield of 13.6 tons/ha, a deep yellow flesh color, and a fruit weight of 1.7kg; The variety with the highest dry matter content of 19.0% was Nhum Xi (SDK-8387, collected in Son La), a high-yield variety, yielding 15.7 tons/ha, a deep orange flesh color, and a weight of 2.5kg.

Figure 3.11. Dry matter content of studied pumpkin varieties
Figure 3.10 shows that most of the seed samples have dry matter content in the range of 6-10%, accounting for 62.12% (82 seed samples), typically Pumpkin (SDK3634), Crow (SDK5355), Pumpkin (SDK7534); group
65
The group with low dry matter content < 6% includes 29 varieties (accounting for 38.28%) typically Pumpkin (SDK3726) (4.0%), Xeng to (SDK7546) (4.0%), Ma uc (SDK8385) (4.7%), Mac uc kho (SDK8393) (5.2%); the group with high dry matter content from 10% includes 21 varieties (accounting for 15.9%) of which 4 varieties have high dry matter content (>13%) including: pumpkin (SDK3630) (13.3%), pumpkin with long fruit (SDK6916) (13.5%), Nhum xi (SDK8387) (19%), Lang qua (SDK19291) (13.5%).
-carotene is a precursor of vitamin A, when entering the body it will be converted into vitamin A to participate in the visual process , besides that it also participates in the metabolism of protein, lipid, glucid and mineral salts . When there is a lack of vitamin A, protein accumulation in the liver will decrease and albumin synthesis in the serum will stop. Lack of vitamin A also increases the phenomenon of kidney stones and reduces potassium in many other organs , one molecule of -carotene will produce two molecules of vitamin A. Thus,
β- carotene is known to play a very important role in the body, studies
Recent studies have shown that β -carotene is also an agent against cancer cells and toxins caused by ultraviolet rays. For the repair of damage caused by ultraviolet rays,
-carotene is even more effective than vitamin A (Gerardus & Grubben, 2004 [48]; Christophe Wiart, PharmD, PhD, 2012) [31].
The evaluation results showed that the studied varieties had an average β-carotene content (5-10 µg/g) of 81.1%. The varieties had a low β-carotene content (< 5 µg/g) of 23.5%. The remaining 13 varieties had a fair level (10 - 15 µg/g) of 9.8% and 2 varieties had a high content (>15
µg/g) accounting for 1.5% are the samples with registration numbers 15164 and 3722.
The vitamin C content of the studied pumpkin varieties ranged from 1.4 - 19.1 mg/100g; the majority of pumpkin varieties (57.6%) had a fairly high vitamin C content (5 - 10 mg/100g), while 40.1% of the varieties had a low level (<5 mg/100g).
The results of the assessment of Vitamin C content shown in Table 3.4 and Figure 3.15 show that: 53 samples (accounting for 40.1%) have a vitamin C content of <5 mg/100g; 67 similar samples (accounting for 50.8%) have a vitamin C content of 5 -
10 mg/100g; 10 varieties (accounting for 7.6%) reached 10 - 15 mg/100g and only 2 varieties (accounting for 1.5%) had very high vitamin C content (>15 mg/100g) which were two pumpkin varieties SDK 7955 (19.1 mg/100 g) and SDK 8396 (17.7 mg/100 g), equivalent to pumpkin varieties F1-LTP 868 [17] or imported Korean pumpkin varieties such as TN220 and Season opener [5].
3.1.4. Identify some promising pumpkin varieties for use as materials
Select and introduce production in the direction of high dry matter content and productivity.
The results of the study on the agrobiological characteristics of a group of 132 pumpkin varieties; analysis of genetic diversity of the gene source, grouping of varieties according to agronomic morphological traits, yield and analysis of the quality of the varieties are the basis for selecting starting materials in the research on breeding and exploiting and developing gene sources with high dry matter content and yield to serve the next research and introduce for production.
The results of the evaluation of the agronomic characteristics of the pumpkin group showed that the pumpkin varieties performed well in this trait but performed normally in another trait. Therefore, in order to select pumpkin varieties with high yield and suitable quality traits, the study used the selection program, version 1.0 of Nguyen Dinh Hien (1996), based on the following criteria: Actual yield, dry matter content, brix, β-carotene content and vitamin C content; with the following selection criteria: varieties with yield ≥ 15.00 tons/ha, dry matter content (%) ≥ 7.5, brix (%) ≥ 5.9, β-carotene content (µg/g) ≥ 8.6 and vitamin C content ≥ 2.50. Five criteria were included in the line selection program, of which 3 criteria (brix, β-carotene content and vitamin C) were not prioritized with a coefficient of 1; 2 priority criteria with a coefficient of 10 were actual yield and dry matter content. Based on the combined criteria, the closest varieties were selected compared to the set target based on the Euclidean distance (this distance did not take into account the relationship between the criteria). The results showed that 14/132 pumpkin varieties met the above selection criteria, presented in Table 3.5 and Appendix 7.
Table 3.5. Selection results of 14 promising pumpkin varieties according to the line selection program (Selection Index)
TT
Registration number | Index | Fruit yield (ton/ha) | Dry matter (%) | Brix (%) | β- carotene (µg/g) | Vitamin C (mg/100g) | |
1 | 8387 | 12.33 | 15.70 | 19.00 | 6.30 | 9.20 | 3.50 |
2 | 5357 | 12.35 | 16.10 | 11.20 | 5.90 | 9.70 | 5.10 |
3 | 15113 | 12.62 | 15.20 | 13.00 | 11.20 | 8.60 | 8.10 |
4 | 3630 | 12.76 | 15.00 | 13.30 | 9.90 | 9.40 | 8.90 |
5 | 5354 | 12.90 | 17.70 | 7.90 | 8.20 | 14.30 | 3.70 |
6 | 15156 | 13.33 | 15.20 | 11.60 | 6.40 | 9.20 | 4.10 |
7 | 8392 | 13.60 | 15.70 | 9.70 | 9.10 | 9.80 | 5.00 |
8 | 3633 | 13.68 | 15.70 | 9.50 | 9.40 | 13.90 | 10.10 |
9 | 8396 | 13.70 | 15.10 | 11.30 | 10.80 | 9.60 | 17.70 |
10 | 3722 | 14.18 | 16.00 | 8.80 | 8.50 | 21.60 | 8.30 |
11 | 16379 | 14.29 | 15.20 | 9.80 | 7.00 | 9.00 | 3.40 |
12 | 3826 | 14.51 | 15.70 | 8.30 | 8.70 | 9.30 | 8.90 |
13 | 9294 | 14.55 | 15.40 | 9.00 | 8.10 | 14.45 | 3.20 |
14 | 6742 | 14.86 | 16.20 | 7.50 | 6.60 | 13.90 | 2.50 |
In which the registration number (SĐK) at the Gene Bank, variety name and collection location are as follows:
TT
Registration number | Generic Name | Collection location | |
1 | 8387 | Pinch | Son La |
2 | 5357 | Pumpkin | Yen Bai |
3 | 15113 | Farmer on the other side | Dien Bien |
4 | 3630 | Pumpkin shell | Thai Nguyen |
5 | 5354 | Eggplant | Lang Son |
6 | 15156 | Pe o | Lai Chau |
7 | 8392 | Slitting | Son La |
8 | 3633 | The result is | Bac Can |
9 | 8396 | Battery | Son La |
10 | 3722 | Pumpkin | Quang Ninh |
11 | 16379 | Farming through | Lao Cai |
12 | 3826 | Pumpkin | Son La |
13 | 9294 | through black | Bac Giang |
14 | 6742 | Pumpkin sticky rice | Lang Son |





