Evaluation of the ability to improve water quality of nitrogen-metabolizing bacteria in the rearing system of black tiger shrimp (Penaeus monodon) - 9



Bacterial density in treatments 2 and 3 was statistically significantly higher than treatment 1 and control from day 10 onwards, which showed that in treatments with added bacteria, the time for bacterial density increase was shortened. This also means that bacteria will quickly play a role in converting organic matter from harmful to beneficial forms.

Some studies by Degrange and Bardin (1995); Huang et al (1989); Balmelle et al (1992) cited by Pham Thi Tuyet Ngan and Nguyen Huu Hiep (2010) showed that the density of nitrifying bacteria in nature is relatively low (from 10 1 - 10 3 CFU/g sludge) and the growth of this group of bacteria is limited by environmental factors such as high NH 3 and pH concentrations; low dissolved oxygen and nitrite, low temperature. In addition, these groups are also limited in development by light intensity and duration.


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Part V

CONCLUSION AND SUGGESTIONS

Evaluation of the ability to improve water quality of nitrogen-metabolizing bacteria in the rearing system of black tiger shrimp (Penaeus monodon) - 9


5.1 Conclusion


- Bacillus bacteria added to the black tiger shrimp rearing system at a density of 10 5 - 10 6 CFU/mL showed better organic decomposition ability than the density of 10 4 CFU/mL and no bacteria added, moreover, Bacillus bacteria have the ability to inhibit the growth of Vibrio bacteria .

- The density of Nitrosomonas and Nitrobacter bacteria in the experiments with added bacteria was relatively stable. The density of these two groups of bacteria in the recirculating system was about 10 times higher than in the water exchange system.

- The survival rate of larvae in the treatments supplemented with bacteria was higher than that of the control, in which NT2 and NT3 (10 5 and 10 6 CFU/mL Bacillus ) were higher and statistically different (p<0.05) compared to the other two treatments. Shrimp reared in the recirculating system had a higher survival rate (PL7 reached 61.1%) than in the water exchange system (PL7 reached 50.1%).

- In experiment 3, COD index in treatments 2 and 3 was statistically lower than treatment 1 and control, while TAN and NO 3 - were statistically higher (p<0.05).

- Vibrio bacteria density in the treatments supplemented with bacteria was statistically significantly lower than in the control treatment.

- Thus, NT2 (10% filter tank volume ratio, bacteria added) showed higher nitrogen conversion efficiency than NT1 and DC but took up less space and cost than NT3.

5.2 Recommendations


Useful bacteria strains such as Bacillus, Nitrosomonas, Nitrobacter should be added to the black tiger shrimp rearing system to improve water quality, shorten the biological filtration operation time, increase the survival rate of larvae, reduce the use of environmental treatment chemicals and antibiotics that have negative effects on the quality of shrimp larvae.


REFERENCES


Aleem, MIH and M. Alexander. 1960. Nutrition and physiology of Nitrobacter agilis . Laboratory of Soil Microbiology, Department of Agronomy, Cornell University, Ithaca, New York.

Austin, B., L.F. Stuckey, PAW Robertson, J. Effendi and DRW Griffith. 1995. A probiotic reducing disease caused by Aeromonas salmonnicida , Vibrio anguillarium and Vibrio ordalii . Journal of Fish Disease, 18: 93-96.

Baticados, MCL, CR Lavilla-Pitogo, ER Cruz-Lacierda, LD de la Pena and

NA Sunaz. 1990. Studies on the chemical control of luminous bacteria Vibrio harveyi and V. splendidus isolated from diseased Penaeus monodon larvae and rearing water. Diseases of Aquatic Organisms, 9: 133-139.

Belgium. 1999. Improved performance of an intensive rotifer culture system by using a nitrifying inoculum (ABIL).

Boyd, C.E. and C.S. Tucker. 1998. Pond Aquaculture Water Quality Management. Kluwer Academic Publishing, Boston, MA, USA. 700pp.

Brigg, MRP, and Funge-Smith, CS 1994. A nutrient budget of some intensive marine ponds in Thailand. Aquaculture Fisheries Management 24, 789 - 811

Bruno, Gomez-Gil, Ana Roque and James F. Turnbull. 2000. The use and selection of probiotic bacteria for use in the culture of larval aquatic organisms. Aquaculture 191, 259-270

Bui Dac Thuyet. 2004. Wastewater treatment from intensive shrimp ponds: biological solutions and research directions. Research Institute for Aquaculture III.

Chanratchakool, P., J.F. Turnbull, S.J. Funge – Smith, I.H. Macrae and C. Limsuwan. 2003. Shrimp health management in ponds. 4th edition. Translators: Nguyen Anh Tuan, Nguyen Thanh Phuong, Dang Thi Hoang Oanh, Tran Ngoc Hai. Danida – Ministry of Fisheries 2003. 153 pages.

Chau Tai Tao. 2005. Research on the application of maturation and larval rearing of black tiger shrimp ( P. monodon ). Master's thesis - Faculty of Aquaculture - Can Tho University.

Chen JC and Chin, TC 1988. Acute toxicity of nitrite to tiger prawn, Penaeus monodon larvae. Aquaculture 69. 253 – 262.

Degrange V. and R. Bardin. 1995. Detection and Counting of Nitrobacter population in soil by PCR. Applied and Environmental Microbiology, June 1995, 2093 – 2098.



Dang Dinh Kim, Nguyen Van Nam, Lai Thi Chi, Tran Van Tua, Nguyen Duc Tho, Dang Thanh Thuy, Le Thu Thuy, Bui Kim Anh, Vu Van Dung and Nguyen Van Quyen. 2006. Research on creating microbial products to treat the bottom of high-yield shrimp ponds. Research Institute for Aquaculture 1.

Dang Thi Hoang Oanh. 2005. General Microbiology Textbook. Faculty of Aquaculture - Can Tho University.

Engel MS and M. Alexander. 1958. Growth and autotrophic metabolism of

Nitrosomonas europaea .

Engel MS, 1958. Morphology of Nitrosomonas europaea and classification of the nitrifying bacteria.

Focht, DDand W. Vertraete, 1997. Biochemical ecology of nitri fication and denitri fication. In: aleander, M. (Ed.), Advances in Microbial Ecology. Plenum Press, New York, pp. 135-214.

Fuller, R. 1989. A review: probiotics in man and animals. J. Appl. Bacteriol.

66:365 – 378

Grommen R., I. Van Hauteghem, M. Van Wambelle, W. Verstraete. 2001. An improved nitrifying enrichment to remove ammonium and nitrite from fresh water aquaria systems.

Grommen R. and Vertraete. 2003. Nitrate removal in aquaria system: use of electrochemically generated hydrogen gas as electron donor for denitrification. In: Proceeding of the 17th Forum for Applied Biotechnology. Communifications in Agricultural and Applied Biological Sciences, Gent 68 (2a), 159 – 162.

Grommen R., Dauw L. and Verstrate W. 2005. Elevated salinity selects for a less diverse ammonia-oxidizing population in aquarium biofilter. In FEMS Microbiology Ecology, Vol.52, Issue 1, pp 1-11

Hastings, JW, and KH Nealson. 1981. The symbiotic luminous bacteria. The Prokaryotes II. Springer – Verlag, New York, 1960 pp.

Herbert, RA, 1999. Nitrogen Cycling in Coastal Marine Ecosystems.

Huys, G. 2002. Preservation of bacteria using commercial cryopreservation systems. Standard Operation Procedure, Asiaresist. 2002.

Jory, ED, 1998. Use of Probiotics in Penaeid Shrimp Grow out Aquaculture Magazine Issue January/February, pp. 62 – 67.

Kristian K. Brandt. 2001. Toxic effects of linear alkylbenzene sulfonate on metabolic activity, growth rate, and microcolony formation of Nitrosomonas and Nitrosospira strains. Applied and Environmental Microbiology, p 2489 – 2498.



Kuan-Fu Liu, Chiu-Hsia Chiu, Ya-Li Shiu, Winton Cheng, and Chun-Hung Liu. 2010. Effects of the probiotic, Bacillus subtilis E20, on the survival, development, stress tolerance, and immune status of white shrimp, Litopenaeus vannamei larvae. Tungkang Biotechnology Research Center, Fisheries Research Institute, Pingtung 928, Taiwan, ROC.

Le Thanh Luu. 2005. Achievements, challenges, orientations and recommendations on science and technology work in aquaculture. Proceedings of the national conference on research and application of science and technology in aquaculture (December 22-23, 2004 in Vung Tau). Agriculture Publishing House, Ho Chi Minh City, pp. 25-39.

Le Tri Tin. 2004. Research on the use of artificial seawater in the production of black tiger shrimp ( Penaeus monodon ) through a biological filtration system. Master's thesis - Faculty of Aquaculture - Can Tho University.

Le Xuan Sinh. 2004. Application of bio-economic model in planning and management of marine shrimp hatchery network in the Mekong Delta. Journal of Aquaculture, CTU. 349 – 361.

Moriarty, DJW 1997. The role of microorganism in aquaculture ponds.

Aquaculture 151, 333-349.

Moriarty, DJW 1998. control of luminous Vibrio species in Penaeid aquaculture ponds. Aquaculture 164: 351-258.

Nguyen Thi Kim Xuan. 2008. Isolation of some strains of Nitrosomonas sp. bacteria capable of reducing ammonia in industrial shrimp ponds. Master's thesis - Institute of Biotechnology Research and Development - Can Tho University.

Nguyen Huu Phuc. 2003. Potential for developing the use of microbial products in aquaculture in Vietnam. Collection of Mekong River fisheries. Scientific report of national conference, scientific research serving aquaculture in the southern provinces. Agriculture Publishing House, Ho Chi Minh City, pp. 194-200.

Nguyen Le Hoang Yen. 2008. Study on the possibility of using ozone in rearing black tiger shrimp ( Penaeus monodon ) larvae. Science Journal – Can Tho University 2008, volume 2, pages 133 – 142.

Nguyen Thanh Phuong, Tran Ngoc Hai, Tran Thi Thanh Hien and Marcy N. Wilder. 2003. Principles and techniques of giant freshwater prawn ( Macrobracium rosenbergii ) seed production. Agricultural Publishing House, 127 pages.

Nguyen Thanh Phuong. 2005. Research on domestication and creation of high quality parent shrimp ( Penaeus monodon ). Ministry-level project, 41 pages.



Nguyen Thanh Phuong. 2007. Research on improving the productivity of giant freshwater prawn larvae rearing using improved green water model. Scientific report. Department of Science and Technology of Can Tho city, 70 pages.

Nguyen Tien Cu, Dang Dinh Kim and Vu Van Dung. 2004. Research on selecting carriers for biofilter applications to treat recirculating aquaculture water. Journal of Fisheries, pp. 18-20.

Nguyen Van Chung, Le Thi Hong, Le Duc Minh, Nguyen Thanh Tung, Tran Van Trong, Ha Le Thi Loc and Nguyen Thi Kim Bich. 1997. Study on the reproductive capacity of black tiger shrimp ( Penaeus monodon Fabricius) from pond culture. Collection of scientific reports of the first national conference on marine biology, 1997, pp. 425 - 430.

Pham Thi Tuyet Ngan, To Cong Tam and Truong Quoc Phu. 2008. The effect of vegetable oil supplementation on the diversity of microbial populations in biological filters. Journal of Science - Can Tho University 2008, volume 1, pages 33 - 43.

Pham Thi Tuyet Ngan and Nguyen Huu Hiep. 2010. Fluctuations in beneficial bacteria density in intensive black tiger shrimp ( P. monodon ) ponds. Scientific Journal - Can Tho University, periodical number 14, 2010, pp. 166 - 176.

Pickering RJ 1975. Analytical Methods – “Nitrifying bacteria (Most – Probable

- Number, MPN, method)”.

Rengpipat, S., W. Phianphak, S. Piyatirativivorakul and P.Menasveta. 1998. Effects of a probiotic bacterium on black tiger shrimp Penaeus monodon survival and growth. Aquaculture 167, pages 301 – 313.

Rennie, RJ and Schmidt EL 1977. Immunoflou-Rescence Studies of

Nitrosomonas and Nitrobacter Population in Soil.

Shariff, M; Yusoff, FM; Devaraja, TN and Srinivasa Rao, PS 2001, The effectiveness of a commercial microbial product in poorly prepared tiger shrimp, Penaeus monodon (Fabricius) in ponds. Aquaculture Research 32, 181-187.

Siriat D., Vichai L., and John DB 2007. Effect of feeding Bacillus sp. as probiotic bacteria on growth of Giant freshwater prawn ( M. rosenbergii de Man). Pakistan Journal of Biological Sciences 10 (9): 1481 – 1485.

Ta Van Phuong. 2006. Application of ozone to treat water and Vibrio spp bacteria in black tiger shrimp larval rearing tanks. Journal of Science – Can Tho University 2006, volume 1, pages 25 – 53.

Tang Minh Khoa. 2008. Research on ozone application to improve the quality of black tiger shrimp ( Penaeus monodon Fabricius) larvae. Master's thesis - Faculty of Aquaculture

- University of Technology



Tang Thi Chinh and Dang Dinh Kim. 2007. Using microbial products in high-yield shrimp farming. Institute of Environmental Technology, Vietnam Academy of Science and Technology.

Thach Thanh, Truong Trong Nghia and Nguyen Thanh Phuong. 1999. Improving and enhancing the efficiency of black tiger shrimp ( Penaeus monodon ) seed production in biofilter systems. Collection of scientific research works, Agriculture part II (pages 185 – 190).

Thach Thanh, Tang Minh Khoa, Tran Nguyen Hai Nam and Nguyen Van Hoa. 2004. Research on the application of artificial seawater in the production of black tiger shrimp ( Penaeus monodon ) through a circulating biological filtration system. Journal of Science - Can Tho University 2004, pp. 248 - 255.

Tran Cong Binh and Truong Trong Nghia. 2002. Useful microorganisms in aquaculture. Special issue of popular science, Ho Chi Minh City Union of Science and Technology Associations, pp. 40-41.

Tran Ngoc Hai and Nguyen Thanh Phuong. 2009. Principles and techniques of farming black tiger shrimp ( Penaeus monodon ). Agricultural Publishing House.

Tran Thi Cam Hong. 2008. Survey on the effectiveness of using probiotics in the actual production of giant freshwater prawn ( Macrobracium rosenbergii) . Master's thesis - Faculty of Aquaculture - Can Tho University.

Tran Thi Kieu Trang, Tran Cong Binh and Truong Quoc Phu. 2006. Determination of ozone threshold for larval and post-larval stages of black tiger shrimp. Journal of Science – Can Tho University 2006, volume 1, pages 241 – 249.

Tran Thi Tuyet. 2008. Isolation of some strains of Nitrobacter sp. with nitrification ability in industrial shrimp ponds. Master's thesis - Institute of Biotechnology Research and Development - Can Tho University.

Trinh Hoai Vu. 2009. Isolation of denitrifying bacteria Pseudomonas stutzeri in wastewater from livestock and aquaculture farms in Soc Trang province. Master's thesis - Institute of Biotechnology Research and Development - Can Tho University.

Vaseeharan, B. and P. Ramasamy. 2002. Cotrol of pathogenic Vibrio spp. by Bacillus subtilis BT23, a possible probiotic treatment for black tiger shrimp ( Penaus monodon ). Applied Microbiology 36, p. 83 – 87.

Vaseeharan, B., J. Lin and P. Ramasamy. 2004. Effect of probiotics, antibiotic sentivity, pathogenicity and plasmid profiles of Listonella anguillarum – like bacteria isolated from Penaus monodon culture systems. Aquaculture 241 (2004), p. 77 – 91.

Verschuere L., Rombaut G., Sorgeloos P., and Verstraete W. 2000. Probiotics bacteria as biological control agents in aquaculture. Microbiology and Molecular Biology Review 64, 655 – 671



Vu The Tru, 1994. Improving shrimp farming techniques in Vietnam. Vietnam Shrimp Farming Association.

Wang Xiang-Hong, Li Jun, Ji Wei-Shang and Xu Huai-Shu. 2003. Application of probiotics in aquaculture. http://www.sciencedirect.com.

Whestone, J.M., G.D. Treece and A.D. Stokes. 2002. Opportunities and constraints in marine shrimp farming. Southern Regional Aquaculture Center (SRAC) application No.2600 USA.

Watson, W.S., E. Bock, H. Harms, H. Koops and AB Hooper. 1989. Nitrite – oxidizing bacteria. In: Bergey's manual of Systematic bacteriology, vol 3. pp. 1810 – 1815.

Weston, DP 1996. Environmental considerations in the use of antibacterial drugs in aquaculture. In: Aquaculture and water resource management. Michael,

CM & Alderman, DJ (Eds.). Paris, Office International des Epizooties: 494-509.

Yassuda K., and N. Taga. 1980. A mass culture method for Artemia salina using bacteria as food. Mer (Bull. Soc. Franco-jap.Océan.ogr.) 18: 53 – 62

Zimmerman RA, LT Bilaniuk, PM Shipkin, DH Gilden and F. Murtagh. 1978. Evolution of cerebral adscess: correlation of clinical features with computed tomography. A case report. Neurology 27 (1): 14 - 19.

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