Mortality Rate (%) of Black Snakehead Fish Fry Using Processed Feed at Different Times

unsuitable, the fish cannot use the provided food, so in the 10-day-old-20% TĂCB/day experiment, the fry showed a very high cannibalism (93.3%). In addition, according to the report of Quin and Fast (1996b), when the prey is a small individual in the school, it will be easier to detect and stimulate the vision than the provided food, and at the same time, they also give the predatory fish more energy/prey. Thus, the fry in the 10-day-old-20% TĂCB/day experiment, although having a much higher growth rate than the other experiments, had the lowest survival rate and the highest cannibalism rate, so it can be seen that the growth of the fry in this experiment was mainly due to cannibalism, not from the use of processed food. On the other hand, the remaining treatments using processed feed had no difference in weight gain and DWG growth ( p>0.05 ) compared to the control (using trash fish), showing that the use of processed feed did not affect the growth of fry.

The results of the study also showed that the specific growth of black snakehead fish in the experiment was higher than that of Bui et al . (2004) on the same subject, which had an SGR of 6.1%/day when weaned at 15 days of age and was also higher than that of Centropomus parallelus when weaned at 35 days of age but the SGR was only 5.53%/day (Alves et al ., 2006). However, the SGR of black snakehead fish is lower than that reported for some other fish species such as sander lucioperca at 18.54%/day and 16.43%/day when introduced to feed on the 19th and 12th day of age (Kestemont et al ., 2007), on basa fish ( Pangasius bocourti) at 20.8%/day when using processed feed on the 2nd day of age (Le et al., 2002) and on Micronema bleekeri at 17.75%/day on the 7th day (Nguyen Van Trieu et al ., 2008).

4.1.6 Mortality rate

During the experiment, the number of dead fish was siphoned and recorded twice a day. Experimental monitoring showed that fish often died in cold weather and died due to disease when the weather changed.

The experimental results are described in Figure 4.7, showing that the highest mortality rate was in the control treatment (32.7%). In the treatments using processed feed, at the same time of weaning, the mortality rate was not significantly different ( p>0.05 ) between the treatments replacing 10% and 20% of TACCB/day, but there was a statistically significant difference ( p<0.05 ) compared to the treatments of weaning at other times.

a

a

a

a

ab

b.c.

c

d

d

40


35


30


Ratioratedie (%)

25


Control

10% TAB/day

20% TAB/day

20



15


10


5


0

10 days old 17 days old 24 days old

Time to practice eating


Figure 4.7: Mortality rate (%) of black snakehead fish fry using processed feed at different times

different feeding points and methods after 5 weeks of experiment.


The creep feeding treatment at 24 days of age had an average mortality rate of 27% and 31%, which was not significantly different ( p>0.05 ) compared to the control treatment and was significantly higher ( p<0.05 ) than the creep feeding treatment at 10 days of age and 17 days of age - 20% TAC/day. Meanwhile, the creep feeding treatment at 17 days of age had a relatively low mortality rate of 19% and 16%, respectively, significantly lower ( p<0.05 ) than the control, but significantly higher ( p<0.05 ) than the creep feeding treatment at 10 days of age. Weaning at day 10 had a significantly lower mortality rate (5.67% and 4.33%) than the other treatments ( p<0.05 ), however, this treatment had a very high cannibalism rate, 79% and 93.3%, respectively. This result is similar to the study on catfish (C. gariepinus ) which showed that under unsuitable nutritional conditions, the mortality rate due to cannibalism would be higher than the natural mortality rate (Hecht and Appelbaum, 1987).

Figure 4.8: Heating the experimental tank system with electric lamps


The results of the study showed that in the experimental treatments of weaning on processed food, the later the weaning time, the higher the mortality rate. The mortality rate of fish in the experiment was mainly due to natural conditions (weather, disease). It was noted during the experiment that in the control treatment or the late weaning treatments (during the time of using trash fish before weaning on processed food), fish were more sensitive to weather and pathogens than the treatments using processed food, so there would be a higher mortality rate than the treatments weaning at an earlier time. This is consistent with the opinion of Nguyen Van Trieu et al. (2008) that the transition from live food to artificial food should be done as soon as possible if it does not affect the survival and growth rate of fry and if fish use artificial food well, it will limit the spread of pathogens from natural food. The results of this experiment are different from the results of a study on Sander lucioperca when they were weaned with a similar method at the age of 12, 19 and 26, with mortality rates of 68.6%; 48.1% and 57.8%, respectively (Kestemont et al., 2007) - which had a fairly high mortality rate compared to black snakehead fish in the experiment and the mortality rate decreased gradually with the time of weaning.

Summary : Based on the results of survival rate, growth rate and cannibalism rate of experiment 1, it shows that the processed food used in this experiment is good food for raising black snakehead fish. This opens up a new prospect in replacing fresh food with processed food in raising black snakehead fish. However, the research results also show that fresh food is indispensable in the rearing process, especially in the first days when the fish start to use external food. The time of use and effective method of feeding processed food for black snakehead fish in the fry stage in this experiment is 17 days old with the method of gradually replacing trash fish with processed food at a rate of 10% TÁCB/day.

4.2 Study on the effects of different attractants on the efficiency of using processed feed of black snakehead fish at the fry stage

At the end of experiment 1, the results showed that the effective time and method of feeding processed food for black snakehead fish fry was 17 days old and the method of gradually replacing trash fish with processed food at a rate of 10% TĂCB/day. Experiment 2 was designed based on the results of experiment 1: fry were fed at 17 days old and the method of replacing 10% TĂCB/day with processed food treatments supplemented with different attractants.

4.2.1 Environmental factors

The physical and chemical factors in the culture environment with overflow water system are presented in Table 4.3.

Table 4.3 : Physical and chemical factors during the experiment


Element

Bright

Afternoon

Temperature ( oC )

27.3±0.36

28.8±0.4

pH

7.7±0.25

7.84±0.27

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NH 3 (mg/l) 0.003 - 0.05

NO 2 - (mg/l) < 0.5


During the experiment, the average daily temperature in the morning was 27.3±0.36 o C and in the afternoon was 28.8±0.4 o C, the temperature fluctuation during the day did not exceed 2 o C. The average daily pH in the morning was 7.7±0.25 and in the afternoon was 7.84±0.27, the pH fluctuation during the day did not exceed

over 0.5. NH 3 content fluctuates between 0.003 - 0.05 mg/l. NO 2 - content is always less than 0.5 mg/l. All these factors fluctuate within the allowable limits for fish growth and development (Truong

Quoc Phu, 2000).


4.2.2 Survival rate

After 4 weeks of experiment, the control treatment (no added attractant) had the lowest survival rate of 61% and was statistically significantly lower ( p<0.05 ) than the remaining treatments (Figure 4.9).

In the experiments with added attractants, the experiments with added fish hydrolysate, squid liver oil and earthworm solution had survival rates of 79.3%, 73.7% and 71.3% respectively, there was no difference between these experiments ( p>0.05 ).

with each other and significantly higher ( p<0.05 ) than the control treatment. The experimental results showed that adding attractant to the feed improved the survival rate of fry.


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90


80


70


Ratioratelive - SR (% )

60


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40


30


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0

Control Fish oil Squid oil Earthworm solution

Solution


Figure 4.9: Survival rate (%) of black snakehead fish fry using processed feed supplemented with different attractants after 4 weeks of experiment.

Previous studies have shown that the complete replacement of natural food with artificial food cannot be implemented in the rearing of most fish species because artificial food does not stimulate fish to catch prey because it does not stimulate fish vision and artificial food does not have an attractive smell (Person le Ruyet et al ., 1993). Fish find it difficult to catch artificial food, so they do not eat enough food (Appelbaum and Damme, 1988). Observations during the experiment showed that in the treatments supplemented with attractants, the survival rate was high, possibly because in these treatments the food had an attractive smell, so when fed, the fish actively caught prey very quickly, limiting the dissolution of the processed food while feeding and increasing the amount of food the fish ate, which was shown in the treatment supplemented with hydrolyzed fish solution. On the contrary, in the control treatment, the fish caught prey more slowly and weakly. This result is similar to that reported in salmon ( Salmo salar ), where attractants improved feeding behavior when fish were transferred from freshwater to saltwater (Toften et al ., 2003).



Figure 4.10: Fish gather on the feeding floor to catch prey (NT2 - fish solution added)


In the experiment, in the treatments using attractants, the treatment supplemented with hydrolyzed fish extract gave a higher survival rate (79.3%) than squid liver oil (73.7%) and earthworm extract (71.3%). Some studies suggest that at the stage of starting to eat outside, the digestive enzymes of fry are not capable of digesting processed food, so exogenous enzymes provided from natural food are necessary for fish at this stage (Dabrowski and Glogowski, 1977; Cahu and Infante, 2001). According to Kotzamanis et al . (2007), adding hydrolyzed protein to the diet, in addition to acting as an attractant, hydrolyzed protein also adds essential amino acids to fry, especially in the stage of weaning on artificial food, these amino acids can replace the amino acids in the species' fresh food. Several other studies on fish hydrolysate have demonstrated that the inclusion of fish hydrolysate in artificial feeds is also considered a method to overcome poor digestibility in fry (Dabrowski, 1984; Govoni et al. , 1986). The results of the experiment are similar to studies on common carp (Carvalho et al ., 1997), turbot ( Solea solea ) (Day et al ., 2008) and sea bass ( Dicentrarchus labrax ) (Kotzamanis et al ., 2007) all had higher survival rates when fish hydrolysate was added to the fish diet.

4.2.3 Growth

Table 4.4 shows that the control treatment had the lowest weight gain (WG) and specific growth (SGR) (0.99g and 7.03%/day, respectively) and was significantly lower ( p<0.05 ) than the remaining treatments. Meanwhile, the treatments supplemented with attractants had higher weight gain and SGR of 1.82g, 1.6g and 1.51g; 8.98%, 8.56% and 8.38%/day, respectively, among these treatments, there was a statistically significant difference ( p<0.05 ). The treatment supplemented with fish solution had the highest weight gain and SGR (1.82g and 8.98%/day) and was significantly higher ( p<0.05 ) than the remaining treatments. Feed supplemented with squid oil resulted in significantly higher weight gain and SGR (1.6 g and 8.56%/day) than the treatment supplemented with earthworm extract and the control, but significantly lower ( p<0.05 ) than the treatment supplemented with fish extract. Using earthworm extract as an attractant resulted in significantly lower weight gain and SGR (1.51 g and 8.38%/day) than the treatment supplemented with fish extract and squid oil, but still significantly higher ( p <0.05 ) than the treatment without attractant.

Table 4.4: Weight growth of black snakehead fish fry using processed feed

with the addition of different attractants before and after 4 weeks of experiment.


Solution

W i (g)

W f (g)

WG (g)

DWG (g/day)

SGR (%/day)

Control

0.16

1.15± 0.04d

0.99±0.04 d

0.035± 0.001d

7.03±0.11 d

Fish hydrolysate

0.16

1.98± 0.03a

1.82± 0.03a

0.065± 0.001a

8.98± 0.05a

Squid liver oil

0.16

1.76± 0.03b

1.60± 0.03b

0.057± 0.001b

8.56± 0.06b

Vermicompost

0.16

1.67±0.03 c

1.51±0.03 c

0.054±0.001 c

8.38± 0.07c

Note: Values ​​in the same column followed by different letters are significantly different (p<0.05). Values ​​shown are mean and standard deviation.


Thus, the addition of attractants to the feed improved the growth of fry. Several previous studies have demonstrated that attractants have the effect of enhancing the palatability of feed for fish, so that the amount of feed used by fish increased, thereby having a positive effect on fish growth (Oliva-Teles et al ., 1999). Several other similar reports on the effects of attractants on Japanese eel (Takii et al ., 1986), on yellowtail ( Sparus aurata) (Tandler et al ., 1982) and sea bass ( Dicentrarchus labrax ) (Gomes et al ., 1997) also showed improvements in feed efficiency and fish growth.

The experimental results showed that the treatment using hydrolyzed fish extract as an attractant had the best growth improvement ability (1.82 g and SGR=8.98%/day). In addition to affecting the ability of hydrolyzed fish extract to

Feed consumption also improves the odor of the feed (Berge and Storebakken, 1996). Fish feed intake increases when the feed is supplemented with hydrolyzed fish extract. In addition, the amino acid content in the feed supplemented with fish extract is higher than that in the unsupplemented feed, thus leading to more efficient protein utilization and faster fish growth (Refstiea et al ., 2004). ) ( Liang et al . , 2006 ) .

4.2.4 Cannibalism ratio

Cannibalism is an inevitable phenomenon in snakehead fish farming. If fish are raised in low nutritional conditions, fish are provided with inappropriate food, then they show great cannibalism (Victor and Akpocha, 1992).

Based on the description of Figure 4.11, it can be seen that in this experiment, the cannibalism rate was quite low, only in the range of 6.67% - 22.33%. This result is because the experiment used an appropriate feeding time (17 days old) and feeding method (10% TĂCB/day), so the fry were able to make good use of the provided processed food. After 4 weeks of experiment, the highest cannibalism rate in the treatment without added attractant (control) was 22.33% and was significantly higher ( p<0.05 ) than the remaining treatments. The treatments with added fish hydrolysate, squid liver oil and earthworm solution had cannibalism rates of 6.67%, 11.33% and 14%, respectively, these rates were significantly lower ( p<0.05 ) than the control treatment (22.33%). In which, the treatment using hydrolyzed fish solution had the lowest cannibalism rate (6.67%), not significantly different ( p>0.05 ) compared to the treatment using squid oil (11.33%), but significantly lower ( p<0.05 ) than the treatment using earthworm solution (14%). The cannibalism rate was not significantly different ( p>0.05 ) between the two treatments using squid liver oil and earthworm solution as attractants.

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