APPENDIX B: EXPERIMENTAL DATA 2
Appendix B.1: Average temperature variation ( o C) in experiment 2
Temperature ( o C) Temperature ( o C)
Day
Morning Afternoon
Day
Morning Afternoon
1
26.8 | 28.3 | 15 | 27.3 | 28.8 | |
2 | 26.9 | 28.4 | 16 | 27.3 | 28.8 |
3 | 27 | 28.5 | 17 | 27.3 | 28.8 |
4 | 27.3 | 28.8 | 18 | 27.3 | 28.8 |
5 | 26.8 | 28.3 | 19 | 27 | 28.5 |
6 | 27 | 28.5 | 20 | 27.3 | 28.8 |
7 | 26.8 | 28.3 | 21 | 27.3 | 28.8 |
8 | 26.8 | 28.3 | 22 | 27.8 | 29.3 |
9 | 27.3 | 28.8 | 23 | 27.9 | 29.4 |
10 | 27.7 | 29.2 | 24 | 27 | 28.5 |
11 | 27.3 | 28.8 | 25 | 27.3 | 28.8 |
12 | 27.8 | 29.3 | 26 | 27.8 | 29.3 |
13 | 27.8 | 29.3 | 27 | 27.5 | 29 |
14 | 26.8 | 28.3 | 28 | 27.8 | 29.3 |
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Appendix B. 2: Average pH variation in experiment 2
pH pH
Day
Morning Afternoon
Day
Morning Afternoon
1
8.2 | 8.4 | 15 | 7.7 | 7.8 | |
2 | 8.1 | 8.3 | 16 | 7.7 | 7.8 |
3 | 8.1 | 8.2 | 17 | 7.5 | 7.6 |
4 | 8 | 8.3 | 18 | 7.6 | 7.7 |
5 | 8 | 8.1 | 19 | 7.4 | 7.6 |
6 | 7.9 | 8 | 20 | 7.6 | 7.7 |
7 | 7.9 | 8 | 21 | 7.4 | 7.5 |
8 | 7.9 | 8 | 22 | 7.4 | 7.5 |
9 | 7.8 | 7.9 | 23 | 7.6 | 7.7 |
10 | 7.9 | 8 | 24 | 7.5 | 7.6 |
11 | 7.8 | 8 | 25 | 7.6 | 7.8 |
12 | 7.8 | 8.1 | 26 | 7.5 | 7.6 |
13 | 7.6 | 7.7 | 27 | 7.4 | 7.5 |
14 | 7.6 | 7.7 | 28 | 7.3 | 7.5 |
Appendix B.3: NH 3 fluctuation (mg/l) in experiment 2
NT 1 NT 2 NT 3 NT 4
1.1 | 1.2 | 1.3 | 2.1 | 2.2 | 2.3 | 3.1 | 3.2 | 3.3 | 4.1 | 4.2 | 4.3 | |
1 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 |
2 | 0.009 | 0.009 | 0.009 | 0.009 | 0.009 | 0.009 | 0.009 | 0.009 | 0.009 | 0.009 | 0.009 | 0.009 |
3 | 0.009 | 0.009 | 0.009 | 0.009 | 0.009 | 0.009 | 0.009 | 0.009 | 0.009 | 0.009 | 0.009 | 0.009 |
4 | 0.003 | 0.009 | 0.009 | 0.003 | 0.009 | 0.009 | 0.009 | 0.003 | 0.009 | 0.003 | 0.009 | 0.009 |
Week
Appendix B.4: NO 2 - fluctuation (mg/l) in experiment 2
NT 1 NT 2 NT 3 NT 4
1.1 | 1.2 | 1.3 | 2.1 | 2.2 | 2.3 | 3.1 | 3.2 | 3.3 | 4.1 | 4.2 | 4.3 | |
1 | <0.5 | <0.5 | <0.5 | <0.5 | <0.5 | <0.5 | <0.5 | <0.5 | <0.5 | <0.5 | <0.5 | <0.5 |
2 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.5 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 |
3 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 |
4 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 |
Week
Note:
NT 1: Control; NT 2: Hydrolyzed fish extract NT 3: Squid liver oil; NT 4: Earthworm extract
56
Appendix B.5: Survival rate, natural mortality rate and cannibalism rate of fish after 4 weeks
Solution | Survival rate (%) | Natural death rate (%) | Cannibalization rate (%) |
1 | 57 | 18 | 25 |
1 | 61 | 17 | 22 |
1 | 65 | 15 | 20 |
2 | 75 | 17 | 8 |
2 | 84 | 11 | 5 |
2 | 79 | 14 | 7 |
3 | 78 | 13 | 9 |
3 | 67 | 18 | 15 |
3 | 76 | 14 | 10 |
4 | 66 | 17 | 17 |
4 | 73 | 15 | 12 |
4 | 75 | 12 | 13 |
Appendix B.6: Weight gain and growth of fish after 4 weeks of experiment | |||
Solution
W i (g) | W f (g) | WG | DWG (g/day) | SGR (%/day) | |
1 | 0.16 | 1,1839 | 1.0239 | 0.0366 | 7,1477 |
1 | 0.16 | 1,1439 | 0.9839 | 0.0351 | 7.0252 |
1 | 0.16 | 1,1139 | 0.9539 | 0.0341 | 6,9301 |
2 | 0.16 | 1,9749 | 1,8149 | 0.0648 | 8,9753 |
2 | 0.16 | 1,9565 | 1,7965 | 0.0642 | 8,9419 |
2 | 0.16 | 2,0079 | 1.8479 | 0.0660 | 9.0346 |
3 | 0.16 | 1,7261 | 1,5661 | 0.0559 | 8,4944 |
3 | 0.16 | 1,7586 | 1,5986 | 0.0571 | 8,5611 |
3 | 0.16 | 1.7845 | 1.6245 | 0.0580 | 8,6132 |
4 | 0.16 | 1,6940 | 1.5340 | 0.0548 | 8,4274 |
4 | 0.16 | 1.6875 | 1.5275 | 0.0546 | 8,4137 |
4 | 0.16 | 1,6318 | 1,4718 | 0.0526 | 8,2939 |
Note:
NT 1: Control; NT 2: Hydrolyzed fish extract NT 3: Squid liver oil; NT 4: Earthworm extract
APPENDIX C: STATISTICAL PROCESSING TABLE
Appendix C.1: Results of statistical processing of experiment 1
ANOVA
Sum of Squares | df | Mean Square | F | Sig. | ||
W f | Between Groups | 11.4 | 6 | 1.89 | 5.5 | 0.004 |
Within Groups | 4.82 | 14 | 0.344 | |||
Total | 16.2 | 20 | ||||
WG | Between Groups | 11.4 | 6 | 1.89 | 5.5 | 0.004 |
Within Groups | 4.82 | 14 | 0.344 | |||
Total | 16.2 | 20 | ||||
SGR | Between Groups | 26.9 | 6 | 4.48 | 6.84 | 0.002 |
Within Groups | 9.18 | 14 | 0.656 | |||
Total | 36.1 | 20 | ||||
DWG | Between Groups | 0.009 | 6 | 0.002 | 5.51 | 0.004 |
Within Groups | 0.004 | 14 | 0 | |||
Total | 0.013 | 20 | ||||
SR | Between Groups | 11897 | 6 | 1983 | 40 | 0 |
Within Groups | 695 | 14 | 49.6 | |||
Total | 12592 | 20 | ||||
TLC | Between Groups | 2387 | 6 | 398 | 14.4 | 0 |
Within Groups | 388 | 14 | 27.7 | |||
Total | 2775 | 20 | ||||
TLAN | Between Groups | 22441 | 6 | 3740 | 90 | 0 |
Within Groups | 582 | 14 | 41.6 | |||
Total | 23023 | 20 |
Note:
W f : Final weight (g) WG : Weight gain (g)
SGR : Relative growth rate (%/day) DWG : Absolute growth rate (g/day) SR : Survival rate (%)
TLC : Mortality rate (%) TLAN : Cannibalism rate (%)
Homogeneous Subsets
Final weight - Wf (g)
NT | N | Subset for alpha = .05 | ||
1 | 2 | |||
Duncan(a) | 5 | 3 | 0.73 | |
4 | 3 | 1.17 | ||
6 | 3 | 1.25 | ||
7 | 3 | 1.26 | ||
1 | 3 | 1.36 | ||
2 | 3 | 1.43 | ||
3 | 3 | 3.22 | ||
Sig. | 0.21 | 1 | ||
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Weight gain - WG (g)
NT | N | Subset for alpha = .05 | ||
1 | 2 | |||
Duncan(a) | 5 | 3 | 0.69 | |
4 | 3 | 1.13 | ||
6 | 3 | 1.21 | ||
7 | 3 | 1.22 | ||
1 | 3 | 1.32 | ||
2 | 3 | 1.39 | ||
3 | 3 | 3.18 | ||
Sig. | 0.21 | 1 | ||
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Note:
NT 1: Control
Treatment 2: 10 days old - 10% feed/day; Treatment 3: 10 days old - 20% feed/day
NT 4: 17 days old - 10% TAD/day; NT 5: 17 days old - 20% TAD/day
NT 6: 24 days old - 10% TAD/day; NT 7: 24 days old - 20% TAD/day
Absolute growth rate - DWG (g/day)
NT | N | Subset for alpha = .05 | ||
1 | 2 | |||
Duncan(a) | 5 | 3 | 0.02 | |
4 | 3 | 0.032 | ||
6 | 3 | 0.035 | ||
7 | 3 | 0.035 | ||
1 | 3 | 0.038 | ||
2 | 3 | 0.04 | ||
3 | 3 | 0.091 | ||
Sig. | 0.21 | 1 | ||
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Specific Growth Rate - SGR (%/day)
NT | N | Subset for alpha = .05 | |||
1 | 2 | 3 | |||
Duncan(a) | 5 | 3 | 8.24 | ||
4 | 3 | 9.64 | 9.64 | ||
6 | 3 | 9.79 | |||
7 | 3 | 9.85 | |||
1 | 3 | 9.97 | |||
2 | 3 | 10.04 | |||
3 | 3 | 12.4 | |||
Sig. | 0.05 | 0.6 | 1 | ||
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Note:
NT 1: Control
Treatment 2: 10 days old - 10% feed/day; Treatment 3: 10 days old - 20% feed/day
NT 4: 17 days old - 10% TAD/day; NT 5: 17 days old - 20% TAD/day
NT 6: 24 days old - 10% TAD/day; NT 7: 24 days old - 20% TAD/day
Survival rate - SR (%)
NT | N | Subset for alpha = .05 | ||||
1 | 2 | 3 | 4 | |||
Duncan(a) | 3 | 3 | 2.33 | |||
2 | 3 | 15.3 | ||||
7 | 3 | 47.7 | ||||
6 | 3 | 56.7 | 56.7 | |||
5 | 3 | 63.7 | ||||
1 | 3 | 64.7 | ||||
4 | 3 | 64.7 | ||||
Sig. | 1 | 1 | 0.14 | 0.22 | ||
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Mortality rate (%)
NT | N | Subset for alpha = .05 | ||||
1 | 2 | 3 | 4 | |||
Duncan(a) | 3 | 3 | 4.33 | |||
2 | 3 | 5.67 | ||||
5 | 3 | 16 | ||||
4 | 3 | 19 | 19 | |||
6 | 3 | 27 | 27 | |||
7 | 3 | 31 | ||||
1 | 3 | 32.7 | ||||
Sig. | 0.76 | 0.5 | 0.08 | 0.23 | ||
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Note:
NT 1: Control
Treatment 2: 10 days old - 10% feed/day; Treatment 3: 10 days old - 20% feed/day
NT 4: 17 days old - 10% TAD/day; NT 5: 17 days old - 20% TAD/day
NT 6: 24 days old - 10% TAD/day; NT 7: 24 days old - 20% TAD/day
Cannibalization rate (%)
NT | N | Subset for alpha = .05 | ||||
1 | 2 | 3 | 4 | |||
Duncan(a) | 1 | 3 | 2.67 | |||
4 | 3 | 16.3 | ||||
6 | 3 | 16.3 | ||||
5 | 3 | 20.3 | ||||
7 | 3 | 21.3 | ||||
2 | 3 | 79 | ||||
3 | 3 | 93.3 | ||||
Sig. | 1 | 0.40 | 1 | 1 | ||
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Note:
NT 1: Control
Treatment 2: 10 days old - 10% feed/day; Treatment 3: 10 days old - 20% feed/day
NT 4: 17 days old - 10% TAD/day; NT 5: 17 days old - 20% TAD/day
NT 6: 24 days old - 10% TAD/day; NT 7: 24 days old - 20% TAD/day