Separation and Determination of Some Forms of Selenium in Seafood Samples
1g Dry sample 6 0 C 24h 50ml 0.5M HCl Centrifuge 2000 (rpm) Decant the above solution. Extract Filter (0.45 µm) Extract 5.5 ml CH 2 Cl 2 shake 15 minutes Organic phase 10ml n-hexane (3 times) Water phase 5ml CH 2 Cl 2 (2 times) Aqueous extract Extract Aqueous Phase shake 5 minutes shake 5 minutes ...


