Home / Acne

Survey on antibiotic resistance of acne-causing bacteria Probionibacterium acnes in Can Tho city - 1


TAY DO UNIVERSITY FACULTY OF PHARMACY - NURSING


Maybe you are interested!


UNIVERSITY THESIS IN PHARMACY

Survey on antibiotic resistance of acne-causing bacteria Probionibacterium acnes in Can Tho city - 1

CODE: 52720401


SITUATION SURVEY

ANTIBIOTIC RESISTANCE OF ACNE-CAUSING BACTERIAPROPIONIBACTERIUM ACNES

IN CAN THO CITY


STUDENT GUIDANCER MSc. DUONG THI BICH PHAM THI TUONG VY

Student ID: 12D720401188 CLASS: UNIVERSITY OF PHARMACY 7B


Can Tho, 2017

ACKNOWLEDGEMENTS

During the process of studying and writing my university thesis, I would like to thank the teachers of the Faculty of Pharmacy and Nursing of Tay Do University for providing me with valuable background knowledge to prepare for my thesis. In particular, I would like to express my deep gratitude to Ms. Duong Thi Bich for her guidance and enthusiastic guidance, helping me overcome difficulties and providing necessary scientific knowledge during the thesis process. Thanks to the participation and comments of teachers, friends and my own efforts, I have completed my university thesis.

I would like to sincerely thank the staff and laboratory staff of Can Tho Dermatology Hospital, Associate Professor, Dr. Huynh Van Ba ​​for creating favorable conditions for me to collect samples and data at the hospital and clinic.

Finally, I would like to thank my family and friends, who have always been with me through difficult and busy times, and have always given me the motivation to complete my graduation thesis well.

Within the framework of the thesis, due to limited time and knowledge, it is inevitable that there will be shortcomings. I look forward to receiving instructions and comments from teachers.

Can Tho, May 19, 2017 Pham Thi Tuong Vy

COMMITMENT TO RESULTS

I hereby declare that this is my own research work. The data and results presented in this thesis are true and have not been published in any previous thesis.

Can Tho, May 19, 2017 STUDENTS


PHAM THI TUONG VY

SUMMARY


Probionibacterium acnes bacteria are considered one of the main causes of acne and the number of drug-resistant bacteria strains is increasing due to the increasing number of antibiotics prescribed in the treatment of acne. Through the topic "Survey of antibiotic resistance of acne-causing bacteria Propionibacterium acnes in Can Tho city" on 100 samples taken from acne patients at Can Tho Dermatology Hospital and Dr. Huynh Van Ba's clinic, 45 strains of Propionibacterium acnes bacteria were isolated. Of the 45 strains of Propionibacterium acnes bacteria surveyed, 45 (100%) were resistant to clindamycin, 32 (71.1%) were resistant to levofloxacin; 40 (88.9%) were resistant to erythromycin; 26 (57.8%) were resistant to cefuroxime; 43 (95.6%) cases were resistant to trimethoprim/sulfamethoxazole; 44 (97.8%) cases were resistant to tetracycline. The resistance rate of Propionibacterium acnes to the 6 antibiotics surveyed was very high. Research on the antibiotic resistance of Propionibacterium acnes helps pharmacists find new, more suitable drugs for the treatment of acne.

Keywords: acne, antibiotic resistance, Propionibacterium acnes.

INDEX

Page

THANK YOU i

COMMITMENT TO RESULTS ii

SUMMARY iii

TABLE OF CONTENTS iv

LIST OF TABLES

LIST OF FIGURES vii

LIST OF ABBREVIATIONS viii

CHAPTER 1. INTRODUCTION 1

CHAPTER 2. OVERVIEW OF DOCUMENTS 3

2.1. CHARACTERISTICS OF ACNE 3

2.1.1. Overview of acne 3

2.1.2. Causes of acne 3

2.1.3. Classification of acne 5

2.1.4. Harmful effects of acne 5

2.1.5. Treatment and prevention of acne 5

2.2. PROPIONIBACTERIUM ACNES BACTERIA 8

2.2.1. Characteristics of Propionibacterium acnes 8 bacteria

2.2.2. Classification of Propionibacterium acnes 9 bacteria

2.2.3. Studies on the treatment of acne-causing bacteria Propionibacterium acnes ..10 2.3. OVERVIEW OF ANTIBIOTICS 10

2.3.1. Introduction to antibiotics 10

2.3.2. Some antibiotics used in the treatment of acne 10

2.4. CURRENT SITUATION OF ANTIBIOTIC USE AND ANTIBIOTIC RESISTANCE 16

2.4.1. The situation of antibiotic use and resistance in the world 16

2.4.2. Situation of antibiotic use and resistance in Vietnam 18

2.5. BRIEF OVERVIEW OF THE STUDY SITE 19

CHAPTER 3. RESEARCH METHODOLOGY 20

3.1. TIME AND LOCATION OF RESEARCH 20

3.2. RESEARCH METHODS 20

3.2.1. Raw materials 20

3.2.2. Experimental tools and equipment 20

3.2.3. Chemicals and environment 20

3.3. RESEARCH METHODS 21

3.3.1. Research design 21

3.3.2. Sample size 21

3.3.3. Patient sample selection criteria 21

3.3.4. Exclusion criteria 21

3.3.5. Research sample collection method 21

3.3.6. Research content 22

3.3.7. Data processing method 26

3.3.8. Research diagram 26

3.3.9. Measures to correct errors 27

3.4. Medical ethics 27

CHAPTER 4. RESULTS AND DISCUSSION 28

4.1. RESULTS 28

4.1.1. Characteristics of acne patients studied in 28 groups

4.1.2. Isolation and identification of Propionibacterium acnes strains from

acne patient skin 31

4.1.3. Results of the study on the ability to inhibit Propionibacterium acnes bacteria

of some antibiotics 42

4.2. DISCUSSION 44

4.2.1. Characteristics of the acne patient group studied 44

4.2.2. Isolation and identification of Propionibacterium acnes strains from skin

acne patient 45

4.2.3. Results of the survey on the ability to inhibit Propionibacterium acnes bacteria

some antibiotics 47

CHAPTER 5. CONCLUSION AND RECOMMENDATIONS 49

5.1. CONCLUSION 49

5.2. PROPOSAL 49

REFERENCES 50

APPENDIX 55

LIST OF TABLES

Table 2.1 Effects of substances used in the treatment of acne 7

Table 2.2 Extracellular products of P.acnes 9

Table 3.1 TYEG medium formula (pH 6.8) 21

Table 3.2 Published sterility standards of antibiotics used 26

Table 4.1 Gender distribution 28

Table 4.2 Age distribution 28

Table 4.3 Distribution by occupation 29

Table 4.4 Distribution by disease severity 29

Table 4.5 Skin condition of acne patients 30

Table 4.6 Clinical manifestations of acne patients 31

Table 4.7 Treatment history of acne patients 31

Table 4.8 Colony characteristics and cell morphology of bacterial strains analyzed

set 33

Table 4.9 Summary of morphological and biochemical characteristics of bacterial strains

set 38

Table 4.10 Culture results of Propionibacterium acnes 40

Table 4.11 Correlation between the results of isolation of bacterial strains

Propionibacterium acnes and oily skin factors 40

Table 4.12 Correlation between isolation results of Propionibacterium acnes strains

and the severity of acne 41

Table 4.13 Correlation between isolation results of Propionibacterium acnes strains

and clinical manifestations of acne patients 41

Table 4.14 Correlation between isolation results of Propionibacterium acnes strains

and treatment history of acne patients 42

Table 4.15 Antibiogram results for P. acnes 43 bacteria

LIST OF IMAGES

Figure 2.1 Normal sebaceous follicle structure 4

Figure 2.2 Structure of sebaceous follicles in acne-prone skin 4

Figure 2.3 Propionibacterium acnes 9 bacteria

Figure 2.4 Structural formula of sulfamethoxazole 10

Figure 2.5 Structural formula of trimethoprim 10

Figure 2.6 Structural formula of erythromycin 11

Figure 2.7 Structural formula of cefuroxime 13

Figure 2.8 Structural formula of tetracycline 14

Figure 2.9 Structural formula of clindamycin 15

Figure 2.10 Structural formula of levofloxacin 15

Figure 3.1 Study diagram 26

Figure 4.1 Acne patient 30

Figure 4.2 Colonies of the isolated bacterial strain on TYEG agar medium supplemented with 0.002% bromocresol purple 32

Figure 4.3 Morphology of bacterial cells under optical microscope 35

Figure 4.4 Catalase test on bacterial isolate 36

Figure 4.5 Testing the ability to produce indole on isolated bacterial strain 36

Figure 4.6 Testing the ability to liquefy gelatin on isolated bacterial strain 37

Figure 4.7 Testing the nitrification reaction ability on isolated bacterial strain 37

Figure 4.8 Graph showing antibiotic resistance rates of P. acnes 43

Figure 4.9 Antibiotic susceptibility test results of P.acnes strain 76N after 48 hours 44

Comment


Agree Privacy Policy *