Home / Growth Ability

B. Effect of Potato, Carrot and Coconut Water Extracts on Shoot Height at Stage 50 – 90 Nsc (Cm)

Potato extract is commonly used in tissue culture. At the same time, in order to test some other types of extracts on the growth of Cattleya orchids, we have added popular subjects in Vietnam such as carrots and coconut water. These extracts have high protein content, which is a valuable food source providing protein, minerals and many different vitamins.

The height results when adding these extracts to the culture medium in table 3.4a show:

At stage 10 – 20 NSC, in terms of extract type, coconut water gave the best results in height, statistically significant, respectively 0.41 cm (10 NSC) and 0.57 cm (20 NSC) but at different concentrations, the difference in shoot height was not statistically significant.

At stage 30 NSC, when changing the concentration levels of the extract, using 10% of the extract gave the best result in terms of height (0.77 cm), although it was not different from the treatment supplemented with 20% of the extract (0.65 cm), but the difference was statistically significant compared to the treatment supplemented with 30% of the extract (0.59 cm). For the type of extract, coconut water gave the best result in terms of height (0.77 cm), which was statistically significant.

At stage 40 NSC, in terms of extract concentration, using 10% extract gave the best result in height (0.85 cm), although not different from the treatment supplemented with 20% extract (0.71 cm), it was statistically significant compared to the treatment supplemented with 30% extract (0.65 cm). When changing the type of extract, coconut water gave the best result in height (0.86 cm), statistically significant.

Table 3.4b Effect of potato, carrot and coconut water extracts on shoot height at stage 50 – 90 NSC (cm)


NSC




Concentration (%)


TB



10

20

30



Potato

0.97

0.55

0.66

0.73 B



Carrot

0.88

0.77

0.71

0.79 B

50


Coconut water

1.10

1.09

0.87

1.02 A


TB

0.98 A

0.80 B

0.75B​


CV = 17.76% F calculated (A) = 6.01 *

F calculated (B) = 9.78 ** F calculated (A x B) = 1.17 ns



Potato

1.11

0.63

0.70

0.81 B



Carrot

0.95

0.86

0.82

0.88B​

60


Coconut water

1.31

1.30

1.10

1.24 A


TB

1.12 A

0.93B​

0.87B​




CV = 19.80% F calculated (A) = 4.01 *

F calculated (B) = 12.78 ** F calculated (A x B) = 1.91 ns


Type of extract

Potato

1.19

0.70

0.79

0.89 B


Carrot

1.03

0.94

0.86

0.94 B

70

Coconut water

1.36

1.42

1.21

1.33 A


TB

1.19 A

1.02 B

0.95B​




CV = 15.59% F calculated (A) = 4.94 *

F calculated (B) = 18.90 ** F calculated (A x B) = 2.29 ns



Potato

1.37

0.84

0.86

1.02 B



Carrot

1.21

1.07

0.88

1.05B​

80


Coconut water

1.63

1.53

1.37

1.51 A


TB

1.41 A

1.15B​

1.04 B




CV = 11.39% F calculated (A) = 17.35 **

F calculated (B) = 36.54 ** F calculated (A x B) =2.41 ns



Potato

1.42

0.96

0.97

1.12B​



Carrot

1.31

1.15

0.92

1.13B​

90


Coconut water

1.78

1.66

1.47

1.64 A


TB

1.51 A

1.26B​

1.12B​




CV = 13.29% F calculated (A) = 11.63 **

F calculated (B) = 26.80 ** F calculated (A x B) = 0.92 ns

Maybe you are interested!

B. Effect of Potato, Carrot and Coconut Water Extracts on Shoot Height at Stage 50 – 90 Nsc (Cm)

Note: Values ​​followed by different letters are statistically significantly different ((**): highly significant; (*): significant; (ns): not significant)

At stage 50 NSC, in terms of concentration, using 10% extract gave the best result in terms of height (0.98 cm), which was statistically significant. Meanwhile, in terms of type of extract, coconut water gave the best result in terms of height (1.02 cm), which was statistically significant.

At stage 60 NSC, when changing the concentration of the extract, using 10% of the extract gave the best result in height (1.12 cm), which was statistically significant. Regarding the type of extract, coconut water gave the best result in height (1.24 cm), which was statistically significant.

At stage 70 NSC, when varying the concentration level, using 10% extract gave the best result in height (1.19 cm), which was statistically significant. While varying the type of extract, coconut water gave the best result in height (1.33 cm), which was statistically significant.

At stage 80 NSC, in terms of concentration, using 10% extract gave the best result in terms of height (1.41 cm), which was statistically significant. In terms of type of extract, coconut water gave the best result in terms of height (1.51 cm), which was statistically significant.

The growth of shoot height continued to increase at the 90 NSC stage. Regarding the concentration factor, using 10% extract gave the best result in height (1.51 cm), which was statistically significant. Meanwhile, regarding the type of extract, coconut water gave the best result in height (1.64 cm), which was statistically significant.

The results showed that the effect on height was very clear at 10% extract. Besides, coconut water also had a very strong effect on height. However, the interaction between extract concentration and type of extract on shoot height was not statistically significant.

Table 3.5a Effect of potato, carrot and coconut water extracts on number of shoots at stage 30

– 60 NSC (buds)


NSC




Concentration (%)


TB



10

20

30



Potato

2.38 cd

0.86 e

1.72 d

1.66 C



Carrot

2.86 bc

2.57 cd

1.98 cd

2.47B​

30


Coconut water

2.70 bc

4.02a​

3.57 ab

3.43 A


TB

2.65

2.48

2.42


CV = 14.35% F calculated (A) = 0.92 ns

F calculated (B) = 54.22 ** F calculated (A x B) = 13.73 **



Potato

2.64 de

0.97f​

1.94 ef

1.85 C



Carrot

4.34b​

3.15 cd

2.77 de

3.42B​

40

Organic matter type

Coconut water

4.00 bc

5.99 a

4.92b​

4.97 A

TB

3.66

3.37

3.21



CV = 12.92% F calculated (A) = 2.46 ns

F calculated (B) = 112.66 ** F calculated (A x B) = 17.08 **


Potato

3.27 cde

1.20f​

2.12 ef

2.19 C



Carrot

4.77 bc

3.98 cd

2.97 de

3.91B​

50


Coconut water

4.44 bcd

8.49a​

5.73b​

6.22 A


TB

4.16 AB

4.56 A

3.60B​




CV = 14.81% F calculated (A) = 5.57 *

F calculated (B) = 99.30 ** F calculated (A x B) = 22.21 *



Potato

3.42 de

1.39f​

2.20 ef

2.34 C



Carrot

5.37 bc

4.52 cd

3.26 de

4.38B​

60


Coconut water

5.36 bc

10.27a​

7.02b​

7.55 A


TB

4.72 AB

5.39 A

4.16B​




CV = 15.71% F calculated (A) = 6.13 **

F calculated (B) = 111.20 ** F calculated (A x B) = 19.57 **

Note: Values ​​followed by different letters are statistically significantly different ((**): highly significant; (*): significant; (ns): not significant)


Stage 10 – 20 NSC, buds have not formed due to short time.


At stage 30 NSC, in terms of extract type, coconut water gave the best results in terms of number of shoots (3.43 shoots), which was statistically significant. Meanwhile, the concentration of 20% coconut water gave the best results in terms of number of shoots (4.02 shoots), although not different from the treatment supplemented with 30% coconut water (3.57 shoots), the difference was statistically significant compared to the remaining treatments.

At stage 40 NSC, in the extracts, coconut water gave the best results in terms of number of shoots (4.97 shoots), which was statistically significant. In addition, the concentration of 20% coconut water gave the best results in terms of number of shoots (5.99 shoots), which was statistically significant compared to the remaining treatments.

At stage 50 NSC, the number of shoots continued to increase. When changing the concentration of the extract, the concentration of 20% gave the best result in terms of the number of shoots (4.56 shoots), which was statistically significant. Regarding the type of extract, coconut water gave the best result in terms of the number of shoots (6.22 shoots), which was statistically significant. Meanwhile, the concentration of 20% coconut water gave the best result in terms of the number of shoots (8.49 shoots), which was statistically significant compared to the remaining treatments.

Statistical analysis results at stage 60 NSC, for extract concentration, 20% concentration gave the best result in terms of number of shoots (5.39 shoots), which was statistically significant. Meanwhile, in terms of extract type, coconut water gave the best result in terms of number of shoots (7.55 shoots), which was statistically significant. In addition, 20% coconut water concentration gave the best result in terms of number of shoots (10.27 shoots), which was statistically significant compared to the remaining treatments.

The height results when adding extracts to the culture medium in table 3.5b show:

At stage 70 NSC, when changing the concentration of the extract, the concentration of 20% gave the best result in terms of the number of shoots (6.52 shoots), which was statistically significant. In addition, in terms of the type of extract, coconut water gave the best result in terms of the number of shoots (8.69 shoots), which was statistically significant. Meanwhile, the concentration of 20% coconut water gave the best result in terms of the number of shoots (12.18 shoots), which was statistically significant compared to the remaining treatments.

At stage 80 NSC, in terms of extract concentration, the concentration of 20% gave the best results in terms of the number of shoots (7.19 shoots), which was statistically significant. In terms of extract type, coconut water gave the best results in terms of the number of shoots (9.66 shoots), which was statistically significant. Meanwhile, the concentration of 20% coconut water gave the best results in terms of the number of shoots (13.22 shoots), which was statistically significant compared to the remaining treatments.

Table 3.5b Effect of potato, carrot and coconut water extracts on number of shoots at stage 70

– 90 NSC (buds)


NSC




Concentration (%)


TB



10

20

30



Potato

3.72 c

1.40 d

2.55 cd

2.56 C



Carrot

6.33b​

5.98b​

3.56 c

5.29B​

70


Coconut water

6.44b​

12.18a​

7.45b​

8.69 A


TB

5.50B​

6.52 A

4.52B​




Organic matter type

CV = 13.18% F calculated (A) = 17.10 **

F calculated (B) = 160.98 ** F calculated (A x B) = 28.46 **


Potato

4.20 c

1.93 d

2.73 cd

2.95 C


Carrot

6.57b​

6.41b​

3.94 cd

5.64B​

80

Coconut water

7.98b​

13.22a​

7.80b​

9.66 A

TB

6.25 A

7.19 A

4.82B​




CV = 14.75% F calculated (A) = 15.80 **

F calculated (B) = 127.43 ** F calculated (A x B) = 16.23 **



Potato

4.55 c

2.14 d

2.87 cd

3.19 C



Carrot

7.04b​

7.47b​

4.03 cd

6.18B​

90


Coconut water

8.11b​

14.06a​

7.89b​

10.02 A


TB

6.57B​

7.89 A

4.93 C




CV = 13.08% F calculated (A) = 27.69 **

F calculated (B) = 147.66 ** F calculated (A x B) = 22.48 **

Note: Values ​​followed by different letters are statistically different ((**): highly significant; (ns): not significant)


Through statistical analysis of the results at the 90 NSC stage, when changing the concentration of the extract, the concentration of 20% gave the best results in terms of the number of shoots (7.89 shoots), which was statistically significant. In addition, in terms of the type of extract, coconut water gave the best results in terms of the number of shoots (10.02 shoots), which was statistically significant. Meanwhile, the concentration of 20% coconut water gave the best results in terms of the number of shoots (14.06 shoots), which was statistically significant compared to the remaining concentrations.

With the appropriate concentration, adding the extract to the environment creates conditions for Cattleya orchids to grow better. The results showed that a concentration of 20% coconut water gave the highest number of shoots.

Table 3.6 Effects of potato, carrot and coconut water extracts on diameter (cm) and weight (g) of shoot clusters at stage 90 NSC


NSC




Concentration (%)


TB



10

20

30



Potato

0.91

0.56

0.60

0.69 B

Diameter (cm)


Carrot

1.08

0.98

0.75

0.94 B

Organic matter type

Coconut water

1.31

1.56

1.13

1.34 A

TB

1.10

1.03

0.82


CV = 24.02% F calculated (A) = 3.26 ns

F calculated (B) = 17.10 ** F calculated (A x B) = 1.35 ns


Potato

0.23 c

0.10 c

0.10 c

0.14 C

Weight (g)


Carrot

0.44b​

0.26 c

0.17 c

0.29 B


Coconut water

0.54b​

0.77a​

0.46b​

0.59 A


TB

0.40 A

0.38 A

0.24B​



CV = 19.76% F calculated (A) = 14.31 **

F calculated (B) = 100.75 ** F calculated (A x B) = 9.41 **

Note: Values ​​followed by different letters are statistically different ((**): highly significant; (ns): not significant)


Statistical analysis of the diameter and mass of the shoot clusters shown in Table 3.6 shows that:

Regarding diameter: when changing the type of extract, coconut water gave the largest diameter (1.34 cm), which was statistically significant compared to the remaining concentrations.

Regarding mass: for the factor of extract concentration, the concentrations of 10% and 20% gave the best mass results (0.40 g and 0.38 g) which were statistically significant. In addition, for the factor of extract type, coconut water gave the largest mass results (0.59 g), which were statistically significant. Meanwhile, the concentration of 20% coconut water gave the largest mass (0.77 g), which was statistically significant compared to the remaining experiments.

Thus, coconut water strongly affects the diameter of Cattleya orchid buds. At the same time, the interaction between extract concentration and type of extract on the mass of buds is very significant. Coconut water concentration of 20% gives the best result in mass of buds.

Experiments show that 20% coconut water concentration is most suitable for the growth of Cattleya orchids.

3.3 Effect of NAA on root formation of Cattleya orchids


For complete seedlings, before being brought to the nursery, the tree must have enough stems and leaves, requiring the tree to have a strong root system, capable of absorbing nutrients for the tree.

According to Nguyen Van Uyen (1989), auxin is a group of growth regulators commonly used in plant tissue culture, which stimulates cell division to form roots. In this experiment, the growth regulator NAA was used at different concentrations to supplement the MS medium to evaluate the effect of the culture medium combined with varying concentrations of NAA on the root formation process of Cattleya orchids.

Table 3.7 Effect of NAA concentration on root formation of Cattleya at stage 20 – 80 NSC (roots/plant)


NAA

(mg/l)




Day after transplanting



20

30

40

50

60

70

80

0

0.00

0.00

0.00 b

0.31 c

0.92 c

1.25 c

1.62b​

1

0.22

0.25

0.71a​

1.09b​

1.51b​

1.67 bc

2.02 ab

2

0.08

0.08

0.22 ab

0.63 c

0.84 c

1.37 c

1.53b​

3

0.05

0.08

0.78a​

1.34 ab

2.14a​

2.38 ab

2.95ab​

4

0.10

0.17

0.64a​

1.57a​

2.26a​

2.56 ab

3.13a​

5

0.22

0.37

0.68a​

1.65a​

2.23a​

2.81a​

3.27a​

CV

F character

10.49%

1.27 ns

14.20%

1.35 ns

15.59%

3.56 *

14.43%

33.94 **

14.08%

23.93 **

18.85%

9.12 **

22.40%

6.34 **

Note: Values ​​followed by different letters are statistically significantly different ((**): highly significant; (*): significant; (ns): not significant)

At stage 20 – 40 NSC, data is converted according to the formula (x+0.5) 1/2


Table 3.7 shows:


Stage 10 NSC, roots have not formed due to short time.


At stage 20 – 30 NSC, roots began to form but the difference was not statistically significant, ranging from 0 – 0.22 roots/plant (20 NSC) and from 0 – 0.37 roots/plant (30 NSC).

Comment


Agree Privacy Policy *